Figure 5
Inhibition of I Ca by CCh. Effect of 10-7 and 10-6 M CCh on atrial ICa from warm-acclimated and cold-acclimated crucian carp were measured using the whole-cell patch-clamp. (Left) A representative recording showing concentration-dependent inhibition of ICa by CCh and partial recovery (R) of ICa upon washout of CCh. Mean results ± SEM from 12 myocytes (from 3 fish) are shown on the right. CCh induced a small but statistically significant increase of outward current, which disappeared upon washout of CCh. The experiments were conducted on atrial myocytes of warm-acclimated crucian carp at 18°C. Asterisk (*) indicates a statistically significant different value (P < 0.05) from the control (C).