Figure 8

Immunoblot analysis of TJ proteins after treatment with MMPs. Occludin appears as a 65 KDa protein (a) and claudin-1 migrates as a 28 KDa band (b). lane 1 = control, no incubation, lane 2 = control, incubation for 1 h at 37°C, lane 3 = incubation with MMP2 for 1 h at 37°C, lane 4 = incubation with MMP9 for 1 h at 37°C. Equal amounts of protein were loaded to each lane (15 μg protein/lane). Antibody against β-actin was used to control the equal amounts of proteins in the lysates (c). No alteration to the tight junction proteins occludin and claudin-1 was observed after incubation with MMPs.