Table 1 Summary of primers designed to amplify kcnq 1-5 and β-actin mRNAs

KCNQ1 fwd

5′- TCC AGT CGC TCA TGT GTC TC -3′

203

KCNQ1 rev

5′- TTT CAT CCC ACC TTC TTT GC -3′

KCNQ2 fwd

5′- GAG CCA GTG CAG GAG AAA AG -3′

344

KCNQ2 rev

5′- TGA GGT AGA AGG CCG ACA CT -3′

KCNQ3 fwd

5′- GAG AAG GAT TCG GCT CAC TG -3′

443

KCNQ3 rev

5′- GCG TCT GCA TAG GTG TCA AA -3′

KCNQ4 (a) fwd

5′-TAT GCA GAC TCC CTC TGG TG-3′

175

KCNQ4 (a) rev

5′-CCT GCA CTT TCA GAG CAA AG-3′

KCNQ4 (b) fwd

5′-GGG CCG CAG GGT TTC TTT AAA CTT-3′

400

KCNQ4 (b) rev

5′-ATG ACA GTA TGC TGC CGT CCT TCA-3′

KCNQ4 (c) fwd

5′-CGG CCG CAG GGT TTC TTT AAA CTT-3′

400

KCNQ4 (c) rev

5′-TCC TTC AGT GGG AAG ATG GGC TTT-3′

KCNQ4 ch19(a) fwd

5′-TGC CTG TAC AAT GTG CTG GAG AGA-3′

265

KCNQ4 ch19(a) rev

5′-AAG GCT TTC TGG CAA AGC GTA GTC-3′

KCNQ4 ch19(b) fwd

5′-ATC AGC CAA TGA TGA CAG ACG GGT-3′

458

KCNQ4 ch19(b) rev

5′-AAG GCT TTC TGG CAA AGC GTA GTC-3′

KCNQ5b fwd

5′- TGC CTG GTA TAT TGG GTT CC -3′

261

KCNQ5b rev

5′- TGA ACC TTC AAG GCA AAA CC -3′

β-actin fwd

5′- TCC CCT TGT TCA CAA TAA CC- 3′

350

β-actin rev

5′- TCT GTG GCT TTG GGA TTC A-3′