Figure 5

Immunohistochemistry demonstrating the cellular distribution of the Kir6.2, SUR1 and SUR2 subunits in cryostat sections of the mouse ventricle. A: Co-staining of mouse ventricular section with smooth-muscle α-actin (green) and anti-Kir6.2 antibodies (Santa Cruz). The latter antibody was detected with Cy-5 conjugated donkey anti-goat IgG (blue). B and C: Double-staining with anti-Kir6.2 antibodies (Santa Cruz) and CAF-1 anti-Kir6.1 antibodies (detected with Cy-3 conjugated donkey anti-chicken IgY (red). The image width of panel A is 109 μm and for panels B and C it is 89 μm. D: Co-staining of a midmyocardial section with anti-SUR1 antibodies (Cy3-conjugated donkey anti-goat secondary antibodies were used; shown in red) and FITC-conjugated smooth muscle anti-actin antibodies (shown in green). E and F: Cryosections stained with a pan anti-SUR2 antibody (secondary antibody was Cy-3 conjugated anti goat IgG. The image widths for panels D-F respectively are 238, 238 and 88 μm.