Figure 1From: Expression and subcellular localization of aquaporin water channels in the polarized hepatocyte cell line, WIF-BRT-PCR for aquaporins and canalicular solute transporters in WIF-B cells. Total RNA from WIF-B cells was reverse transcribed using random primers and then PCR-amplified with primers designed to amplify a nonconserved region of each AQP and solute transporter cDNA. Isolated rat hepatocyte cDNA were used as positive controls. Lane 1, negative control; lane 2, positive control; lane 3, WIF-B cells.Back to article page