Figure 1

Determination of NO release after treatment of hepatocytes with bradykinin. Fluorescence intensity was measured after 2 h incubation: with 10 μM DAF-2DA in basal conditions (white column, reference), in presence of 0.01 mM bradykinin (hatched column), in presence of 0.1 mM bradykinin (crosshatched column) and in presence of 0.1 mM bradykinin with 1.68 mM L-NAME (black column). The excitation wavelength was 495 nm and the emission wavelength was 515 nm. Values, expressed as a percentage of control values, are the means ± S.E.M. (bars) of four independent experiments. p < 0,05 compared with control