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Table 2 Effect of Media Composition on MDCK Barrier Function. Confluent MDCK cultures were placed in one of the indicated treatment groups for 24 hours. Antimycin A (10 μM) was added at 21 hours following onset of experiment. The mean TER and fluorescein flux ± SE is reported from four independent experiments. ANOVA was performed followed by a Bonferroni post test with all comparisons against the cells in media containing 5% FBS and glucose, *P < 0.01.

From: Proinflammatory cytokines tumor necrosis factor-α and interferon-γ modulate epithelial barrier function in Madin-Darby canine kidney cells through mitogen activated protein kinase signaling

Glucose

+

-

-

+

-

Serum, 5%

+

-

-

+

-

IFNγ, ng/ml

-

-

-

60

60

TNFα, ng/ml

-

-

-

30

30

Antimycin A, 10 μM

-

-

+

-

-

TER, Ω*cm2

72.3 ± 0.5

62.2 ± 2.5

7.1 ± 0.3*

162.2 ± 3.5*

105.0 ± 4.9*

Fluorescein Flux, AU

247.9 ± 8.5

1198.2 ± 52.1*

9201.7 ± 250.5*

2005.3 ± 50.5*

2440.3 ± 45.6*