Figure 3

L-arginine stimulates insulin secretion in various cell lines incubated with the human insulin adenovirus. A. HepG2, B. NIH 3T3, and C. HeLa cells incubated with the human insulin adenovirus were transferred to 1 mM glucose overnight and washed with KRB buffer. After addition of 25 mM glucose or 1 mM glucose with or without 20 mM L-arginine and 100 μM L-NNA, insulin release was measured in the medium and is expressed as fold-difference over insulin secretion at 1 mM glucose.