Figure 3

PAR4-AP induces bladder inflammation. Anesthetized female C57BL6 mice were catheterized, the bladder was emptied, and a volume of 200 μl of a solution of PAR4-AP (10 μM) was instilled into the urinary bladder. Twenty four hours later, bladders were removed, processed for histology, and stained with H&E. At low magnification, photomicrograph illustrates the distribution of inflammatory cells extending from the submucosa to deep regions in the detrusor smooth muscle (green arrows). Magnifications = ×100