Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans
© Lagido et al; licensee BioMed Central Ltd. 2008
Received: 18 December 2007
Accepted: 02 April 2008
Published: 02 April 2008
The ATP levels of an organism are an important physiological parameter that is affected by genetic make up, ageing, stress and disease.
We have generated luminescent C. elegans through ubiquitous, constitutive expression of firefly luciferase, widely used for in vitro ATP determination. We hypothesise that whole animal luminescence reflects its intracellular ATP levels in vivo. To test this, we characterised the bioluminescence response of C. elegans during sublethal exposure to, and recovery from azide, a treatment that inhibits mitochondrial respiration reversibly, and causes ATP depletion. Consistent with our expectations, in vivo luminescence decreased with increasing sublethal azide levels, and recovered fully when worms were removed from azide. Firefly luciferase expression levels, stability and activity did not influence the final luminescence. Bioluminescence also reflected the lowered activity of the electron transport chain achieved with RNA interference (RNAi) of genes encoding respiratory chain components.
Results indicated that C. elegans luminescence reports on ATP levels in real-time. For the first time, we are able to directly assess the metabolism of a whole, living, multicellular organism by determination of the relative ATP levels. This will enable genetic analysis based on a readily quantifiable metabolic phenotype and will provide novel insights into mechanisms of fitness and disease that are likely to be of relevance for other organisms, as well as the worm.
The physiology of model organisms is often less well understood than their genetics (phenotypic gap), but is essential to the understanding of gene function. We argue that the ATP levels of an organism are a relevant physiological parameter that is amenable for phenotypic analysis. Perturbation of an organism's ATP levels is an early indication of metabolic and physiological effects of disease, genetic make-up and stress. For example, one of the first manifestations of Alzheimer's is a decrease in energy metabolism in parts of the brain that correlates with a specific decrease in the activity of a key mitochondrial electron transport chain (ETC) enzyme, cytochrome c oxidase . Deficiencies in energy metabolism are associated with other age-related metabolic and neurodegenerative diseases [2, 3]. Impaired ATP production is a feature of Friedreich's ataxia  and Parkinson's disease (PD) [5, 6]. A systemic defect in mitochondrial complex I is implicated in PD, and its pathological features have been reproduced by chronic exposure of mice to rotenone, a pesticide that specifically inhibits complex I .
The "steady state" ATP levels of an organism also reflect genetic factors. Knockdown of ETC genes in C. elegans resulted in decreased in vitro ATP levels , and deletion of genes in the insulin signalling pathway resulted in higher ATP levels [8, 9]. In addition, there is an environmental influence, with exposure to stress, such as heat-shock , metabolic and oxidative stress [11–13], anoxia , and starvation [11, 15, 16] shown to decrease ATP levels and increase the AMP: ATP ratio.
Stress induced changes in ATP concentrations are often rapid and transient. Depending on the severity of stress, compensatory mechanisms aimed at restoring ATP levels are quickly activated . Therefore stress responses are better studied in real-time. We describe here a novel sensor for real-time in vivo assessment of relative ATP levels in C. elegans.
We modified C. elegans to express firefly luciferase constitutively and ubiquitously throughout development. The enzyme is widely used in vitro to report on ATP levels: it catalyses the oxidation of luciferin in a reaction that consumes ATP and generates light and AMP . Because C. elegans is transparent the luc-marked strains emit light when provided with exogenous luciferin. We have previously demonstrated a link between light levels and the worm's health upon exposure to environmental stress . In this study, we generated highly luminescent strains of C. elegans that contained a luciferase gene, luc+, fused to the green fluorescent protein (GFP) gene, and specifically addressed the hypothesis that whole animal luminescence reflects its intracellular ATP levels in vivo. To test this hypothesis we characterised the bioluminescence response of C. elegans during sublethal exposure to, and recovery from sodium azide (NaN3), a treatment that inhibits mitochondrial respiration reversibly, and causes ATP depletion . Consistent with our hypothesis, in vivo luminescence decreased with increasing sublethal azide concentration, and recovered fully when azide was removed. We then targeted mitochondrial respiratory chain components by RNAi, and have shown a decrease in luminescence that was consistent with the drop in in vitro ATP reported by Dillin et al. .
Although previously achieved at the single cell level [20, 21], this is the first report to show that luminescence can be used for assessment of ATP in a living multicellular organism. This will enable genetic analysis based on a readily quantifiable metabolic phenotype and has the potential to further our understanding of the many genetic pathways involved in diverse aspects of C. elegans physiology, such as metabolism, ageing, disease and stress response. Since many of the C. elegans genes are well conserved , the significance of these findings will extend well beyond this organism.
Strain characterisation and effect of sodium azide (NaN3) exposure
In vitro determination of luciferase levels
To control for potential effects of NaN3 on luciferase protein levels, or its function, luciferase activity was measured in lysates of worms that were exposed to azide under the same conditions as for in vivo experiments (Figure 2D). A commercial kit that provided saturating levels of exogenous ATP and luciferin was used for this purpose. Luciferase activity was normalised to protein content of sample. No significant trend was observed between luciferase activity and NaN3 concentration (regression coefficient not significantly different from zero; P = 0.623466), therefore, under our experimental conditions the level and activity of luciferase did not account for the reduced light output in response to NaN3 (Figure 2D).
Recovery from 30 min exposure to azide
RNAi knockdown of respiratory chain genes
In C. elegans, assessment of ATP levels has until now been carried out in vitro [7–9, 23], a method which is accurate but implies the destruction of worms, limits the scalability of the analysis and cannot be carried out in real-time. We report on the construction of a luminescent transgenic C. elegans strain and have tested the hypothesis that light emitted by living C. elegans is a reflection of its ATP pools. We used two approaches: i) exposure to and recovery from sodium azide, a specific mitochondrial inhibitor, and ii) RNAi towards genes that are essential for mitochondrial function. Azide inhibits complex IV of the mitochondrial respiratory chain by binding reversibly to cytochrome c oxidase , this arrests the flow of electrons and leads to a decrease in ATP synthesis. Because the inhibition is reversible azide has been widely used as a C. elegans anaesthetic . Changes in bioluminescence upon exposure to, and recovery from, sublethal concentrations of azide were consistent with reversible ATP depletion caused by azide. Recovery in bioluminescence occurs within 30 min of removing worms from azide. Similarly, recovery of ATP depletion resulting from anoxia occurs within 45 min of reversal of anoxia . Luciferase expression and activity levels stayed constant in the azide experiments and therefore did not contribute to observed changes in bioluminescence.
The substrate luciferin has to be present intracellularly for light emission. It is not known how luciferin enters C. elegans. It is possible that it passes through the permeabilised cuticle. Alternatively, it may be ingested through the pharynx. If an active pharynx was a requirement for luciferin entry, then substrate availability could be a limiting factor for luminescence under experimental conditions that alter the pumping rate, such as toxin exposure . However, in this work, although exposure to azide stopped pharynx activity, the worms were able to emit light when provided with the substrate, indicating that luciferin was able to cross the permeabilised cuticle. Luciferin has been deemed to be poorly taken up by cells , however, empirical data suggest that luciferin uptake occurs readily. Luciferin crossed the blood-brain barrier in mice easily, passing through endothelial cells that are amongst the least permeable cells in the mouse . Studies in other organisms such as Drosophila , zebra fish  and Arabidopsis  also indicated that diffusion and permeability of luciferin did not limit bioluminescence. Furthermore, we have observed that luciferin was capable of crossing the shell of unhatched C. elegans embryos, considered poorly permeable to chemicals, resulting in bioluminescence. We have also captured images of widespread luminescence in the worm's tissues, contrary to what would be expected if luciferin was poorly taken up by cells.
Inhibition of respiratory chain components by RNAi provided a means of depleting ATP levels without exogenous chemicals. The response measured by bioluminescence is in agreement with in vitro data. In vitro ATP was reduced to 20–40% when cyc-1 or atp-3 were knocked down, and to 40–60% for cco-1 RNAi . All experimental data were consistent with the hypothesis that C. elegans luminescence reflects its ATP pools. Additionally, the RNAi experiments illustrate bioluminescence as a phenotype that could be the basis for genetic analysis.
Wildtype firefly luciferase is targeted to peroxisomes  and has a 3 h half-life [27, 33]. In this study, the nematodes were transformed with a modified firefly luciferase gene luc+ fused to gfp, which is not targeted to the peroxisomes. Luc+ is expressed in the cytosol and has a half-life of 10 h in human breast cancer cells . The half-life of GFP is 26 h  and therefore should not contribute to instability of the fusion protein. The greater stability of Luc+ is an advantage for studies where ATP changes may be tracked over time, as opposed to the requirement for a short half-life when luciferase reports on gene expression.
One critical aspect that will affect the luminescence readings is the levels of firefly luciferase present, this may vary for example between different strains and developmental stages. Hence, the exact relationship between light output and ATP concentrations will depend on the experimental conditions, ruling out precise determination of ATP content. We propose this strain as a relative sensor of ATP levels which can be applied to interrogate mitochondrial function and metabolism of living worms in a non-destructive, real-time and scalable manner. This will have broad appeal as sublethal physiological parameters are often difficult to quantify, especially on a large scale. Perhaps the most exciting developments will be the identification of novel genes and pathways underlying physiological response, as well as a better understanding of classical pathways. The integrated luc+:: gfp fusion we described can be crossed into available strains carrying gene deletions or into new mutants generated by mutagenesis. Alternatively, any of the worm's genes can be targeted for inactivation by RNAi and bioluminescence will provide an easily quantifiable metabolic phenotype. The luciferase gene may also be placed under promoters that will drive its expression in specific tissues and allow for relative assessment of ATP levels in that tissue. Ballistic transformation methods will enable expression of the transgene in the germline where required. The transgenic strains described here offer a unique opportunity to explore the links between physiology and genetics of C. elegans and many other organisms with which it shares homologue genes, including humans .
We have described the construction of highly luminescent strains of C. elegans and have shown that emitted light is a reflection of their ATP pools. Changes in bioluminescence upon exposure to, and recovery from, sublethal concentrations of a respiratory inhibitor were consistent with the levels of ATP determined in vitro for similar conditions. Luciferase activity levels remained stable throughout and consequently did not contribute to the measured changes in bioluminescence. We also presented evidence that luciferin crossed the permeabilised worm cuticle.
We used RNAi towards ETC components to reduce ETC activity and lower ATP synthesis. For the first time mitochondrial function of a whole multicellular organism was determined by in vivo luminescence, with light levels providing a sensitive measure of the ATP levels. Thus, luminescence proved an efficient means for detection of sublethal energy related RNAi phenotypes.
Deciphering gene function is an important challenge in the post-genomic era. Often genes conferring morphological traits are the easiest to study, whereas those involved in sublethal behaviour and physiology prove hardest. The ATP levels of an organism often provide an early manifestation of changes induced by stress and mitochondrial diseases. Our strains will enable rapid real-time assessment of relative ATP levels of a living model organism and will further the research of its biology.
Construction of plasmid pSLGCV
The backbone for pSLGCV was plasmid pPD95.79 (Firelab, Carnegie Institution for Science), which contains gfp. To this backbone we added sequentially the sur-5 promoter , PCR-amplified from plasmid pTG96 and then the luciferase gene PCR-amplified from vector pSP-luc+ (Promega). A 3.7 kb fragment upstream of the sur-5 gene containing the promoter, was amplified using primers 5'- ATAAGCTTGCATGCCTGCATTGC-3' and 5'-AGACACCCCGGGCTTTCTGAAAAC-3' (this introduces a Sma I site and removes the start codon for the sur-5 gene). The sur-5 promoter was inserted in the pPD95.79 backbone using appropriate restriction sites (Sph I and Sma I).
The luc+ gene was amplified using primers 5'-TCCCGGGAAGCTTTCCATGGAAGAC-3' and 5'-CTAGGGTACCACGGCGATCTTTCC-3'. Sma I and Kpn I were used to insert luc+ downstream of the sur-5 promoter and upstream of and in frame with gfp. The peroxisome tagging sequence is absent from luc+ and the NLS for sur-5 was excluded from pSLGCV, so that LUC+::GFP was expressed in the cytoplasm.
Microinjection of the gonad syncytium was carried out as standard . The plasmid pSLGCV was co-injected with the marker pRF4 at 250 ng/ml each. A transgenic line, feEx44 [sur-5::luc+::gfp; rol-6(su1006)], was isolated and the array was integrated by EMS mutagenesis , followed by selection of F2 animals that gave rise to 100% roller progeny. We obtained two independently integrated lines, the first into the X chromosome and the second where the transgene appears to be in chromosome V. The first line was out-crossed 8 times with the wild-type N2 strain to generate PE255 (feIs5), and the second was out-crossed 10 times to generate PE254 (feIs4). The data shown were obtained with feIs5, except where otherwise stated.
Measurement of luminescence
Luminescence was measured in a Clarity microplate luminometer (Biotek) in the visible spectral range between 300 and 600 nm, (firefly luciferase typically emits at 550–570 nm). White microplates were used (Greiner) with approximately 100 worms per well (in 100 μl). An automated dispenser delivered 50 μl of luminescence buffer to each well, consisting of citrate phosphate buffer pH 6.5, 0.1 mM D-luciferin, 1% DMSO and 0.05 % triton-X (all final concentrations). Luminescence of the PE255 strain increases steeply in the first minute after adding luciferin, followed by a slower increase to its maximum levels observed within the second minute. The light levels remain fairly stable during the first 5 minutes, followed by a gradual decrease to 60–80% of the maximum luminescence values in the first half hour (Additional file 1). Luminescence was read for 1 sec, 5 min after adding luciferin, except for the azide recovery experiments, where incubation with luciferin was only 3 min. These incubation periods allowed for maximum luminescence to be reached. During incubation with luciferin plates were shaken at 160 rpm. Except where otherwise mentioned, all luminescence measurements were carried out in vivo.
Measurement of fluorescence
Fluorescence was quantified in a FLUOstar OPTIMA microplate reader (BMG labtech) using 485 nm excitation filter and a 520 nm emission filter. Background measurements were subtracted from readings.
Sodium azide (NaN3) exposure bioassays
Worm cultures were synchronised by bleaching and overnight hatching in M9 . L1 stage nematodes were washed and incubated (20°C, 160 rpm) for 45 h in S medium supplemented with 15 g/L E. coli OP50, at a density of 20 to 30 worms per 10 μl. 50 μl of the worm culture was aliquoted to each well of 96-well plates, white for luminescence or black for fluorescence. 50 μl of sodium azide (SIGMA) was added per well to final concentrations of 0, 1, 2.5, 5, 10 and 15 mM. Each concentration was tested on 8 replicate wells. The plates were covered with air permeable seals (ABgene), and incubated at 20°C, 160 rpm, for 30 min prior to measurement of light output or fluorescence. To assess survival worms exposed to each NaN3 concentration were pooled and washed 3 times with S basal , supplemented with 0.01% Tween-20 to prevent worms from sticking to plasticware. Triplicate 10 μl samples were plated on to NGM plates  and viability assessed by motility within 4 h.
Pharyngeal pumping assay
Worms were treated as described in NaN3 exposure bioassays. An adaptation of the method of Mörck et al.  was used for assessing pharyngeal pumping. Briefly, after exposure to the various azide concentrations, Fluoresbrite™ Polychromatic red microspheres, 0.5 μm diameter (Polyscience, Inc), suspended in luminescence buffer, were added to each well, to a final dilution of 50 times. After 10 min contact time, worms were washed with M9 plus 1 mM levamisole (600 × g, 2 min), transferred to 2% agarose (w/v in M9) pads and mounted for observation under fluorescent microscopy (Texas red filters). A minimum of 30 worms were scored for the presence of microspheres in their pharynx and gut, for each of the azide concentrations tested.
Recovery from azide
Synchronised worms were exposed to 10 mM NaN3 for 30 min, in S complete supplemented with E. coli OP50. Luminescence was read just prior to washing worms from NaN3, and this reading was adopted as the zero time point for recovery (t = 0). Recovery was timed from removal of azide solution. Worms were then washed once, centrifuged (1 min, 600 × g) resuspended in S complete with 7.5 g/L E. coli OP50, and aliquoted to 96-well plates. Luminescence was measured at different time points, as quickly as possible, during the first half hour of recovery. At each time point, a different set of samples was read, so that the incubation time with luciferin was the same (3 min) each time. Each luminescence time point is the average of 24 replicate wells. Luminescence of worms not exposed to azide was taken as the guideline for full recovery.
In vitro luciferase assay
Exposure to 10 mM azide was carried out as described above. Worms were then washed 3 times (1 min, 600 × g) in S basal, and approximately 1000 worms in 100 μl of S basal were frozen in liquid nitrogen and stored at -80°C until analysis. Four replicate samples for each azide concentration were thawed on ice and 200 mg glass beads (212–300 μm) and CCLR reagent were added (Promega). To obtain worm lysates samples were put through a Fastprep homogeniser 8 times (5000 rev/min, 30 s) with incubation on ice in between runs. Lysates were analysed using Luciferase Assay system (Promega), according to manufacturer's instructions. The in vitro luminescence data were normalised to protein content of samples.
In vitro ATP determination
A luminometric method adapted from Braeckman et al.  (Braeckman pers. comm.) was used. Briefly, four replicate samples of washed worms were collected in 100 μl of S basal, quickly frozen in liquid nitrogen and stored at -80°C. Worms were broken up by disruption with glass beads (212–300 μm) in a Fastprep homogeniser after addition of 8 % (v/v) HClO4. Extracts were neutralised with 1.3 M KHCO3, and buffered with 1 M K phosphate buffer (pH 7.6) prior to measurement of ATP concentrations with the ATP bioluminescence CLS II kit (Roche), in a Clarity luminometer (Biotek). ATP concentrations were normalised to protein content of samples.
BCA kit (Pierce) was used in all protein determinations according to manufacturer's instructions, with incubation at 60°C.
RNAi Knockdown of respiratory chain genes
The Rivers et al. protocol for RNAi feeding in liquid culture was used . Approximately 15 synchronised L1 nematodes were placed in each well of 96 -well microplates and cultivated with bacteria expressing dsRNA for respiratory chain genes (MRC GeneService clones), or control clones (GFP clone: pPD128.110; empty vector control: pPD129.36; both Firelab vectors), for 4 days at 25°C without shaking, prior to measurement of in vivo luminescence (as above) and length. To control for reduced body size, luminescence readings were normalised to average worm length. A microscope equipped with an ocular micrometer was used to measure worms after immobilisation with levamisole (200 μM). At least 30 measurements were taken for each experimental condition.
Imaging of worms
A Photometrics CASCADE II 512 Air cooled (-70°C) CCD sensor and a Deltavision microscope were used to capture images of the glp-4(bn2); feIs5 strain. Worms were picked into 5 μl of luminescence buffer plus 200 μM levamisole (to immobilise worms) and mounted in 2% agarose pads. Luminescence was captured with no incident light, in a dark chamber, with 10 sec integration, 18 min (± 2 min) after treatment with the luminescence buffer plus 200 μM levamisole. As a control, conditions for imaging luminescence were tested on worms that carry the plasmid PTG96  and display expression of GFP on its own. No luminescence was captured for the control. For imaging fluorescence illumination through FITC filters was used with an exposure time of 0.2 seconds. A Zeiss Axioplan2 microscope with a HAMAMATSU ORCA-ER CA742–80 digital camera was used for additional photographs of worms, including those of the feIs5 strain and the pharyngeal pumping assay.
Statistical analysis was performed with SPSS 15.0 and Microsoft Office Excel 2003. Least significant differences (LSD)  were calculated for statistically significant ANOVA results.
List of abbreviations used
electron transport chain
Green fluorescent protein
nuclear localisation signal.
We thank: Firelab (Carnegie Institution for Science) for provision of plasmids pPD95.79, pPD128.110 and pPD129.36; John Yochem and Min Han for plasmid PTG96; Valerie Undrill for assistance with construction of plasmid pSLGCV; Debbie McLaggan for assistance with Figure 3C experiment, and critical reading of the manuscript; Kevin MacKenzie for help with capturing Figure 1A image; Bart Braeckman for sharing the protocol for the in vitro determination of ATP, and critical reading of the manuscript; Fundação para a Ciência e a Tecnologia (PRAXIS XXI, Portugal) for financial support (to CL) during generation of the feEx44 strain. This work was supported by The Leverhulme Trust (Project ID20040407). Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, funded by the NIH National Center for Research Resources (NCRR).
- Valla J, Berndt JD, Gonzalez-Lima F: Energy hypometabolism in posterior cingulate cortex of Alzheimer's patients: superficial laminar cytochrome oxidase associated with disease duration. J Neurosci. 2001, 21 (13): 4923-4930.PubMedGoogle Scholar
- Petersen KF, Befroy D, Dufour S, Dziura J, Ariyan C, Rothman DL, DiPietro L, Cline GW, Shulman GI: Mitochondrial dysfunction in the elderly: possible role in insulin resistance. Science. 2003, 300 (5622): 1140-1142. 10.1126/science.1082889.PubMed CentralView ArticlePubMedGoogle Scholar
- Wallace KB, Starkov AA: Mitochondrial targets of drug toxicity. Annu Rev Pharmacol Toxicol. 2000, 40: 353-388. 10.1146/annurev.pharmtox.40.1.353.View ArticlePubMedGoogle Scholar
- Ventura N, Rea SL, Testi R: Long-lived C. elegans mitochondrial mutants as a model for human mitochondrial-associated diseases. Exp Gerontol. 2006, 41 (10): 974-991. 10.1016/j.exger.2006.06.060.View ArticlePubMedGoogle Scholar
- Milakovic T, Johnson GV: Mitochondrial respiration and ATP production are significantly impaired in striatal cells expressing mutant huntingtin. J Biol Chem. 2005, 280 (35): 30773-30782. 10.1074/jbc.M504749200.View ArticlePubMedGoogle Scholar
- Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT: Chronic systemic pesticide exposure reproduces features of Parkinson's disease. Nat Neurosci. 2000, 3 (12): 1301-1306. 10.1038/81834.View ArticlePubMedGoogle Scholar
- Dillin A, Hsu AL, Arantes-Oliveira N, Lehrer-Graiwer J, Hsin H, Fraser AG, Kamath RS, Ahringer J, Kenyon C: Rates of behavior and aging specified by mitochondrial function during development. Science. 2002, 298 (5602): 2398-2401. 10.1126/science.1077780.View ArticlePubMedGoogle Scholar
- Houthoofd K, Braeckman BP, Lenaerts I, Brys K, Matthijssens F, De Vreese A, Van Eygen S, Vanfleteren JR: DAF-2 pathway mutations and food restriction in aging Caenorhabditis elegans differentially affect metabolism. Neurobiol Aging. 2005, 26 (5): 689-696. 10.1016/j.neurobiolaging.2004.06.011.View ArticlePubMedGoogle Scholar
- Houthoofd K, Fidalgo MA, Hoogewijs D, Braeckman BP, Lenaerts I, Brys K, Matthijssens F, De Vreese A, Van Eygen S, Munoz MJ, Vanfleteren JR: Metabolism, physiology and stress defense in three aging Ins/IGF-1 mutants of the nematode Caenorhabditis elegans. Aging Cell. 2005, 4 (2): 87-95. 10.1111/j.1474-9726.2005.00150.x.View ArticlePubMedGoogle Scholar
- Findley RC, Gillies RJ, Schulman RG: In vivo phosphorus-31 nuclear magnetic resonance reveals lowered ATP during heat shock of Tetrahymena. Science. 1983, 219 (4589): 1223-1225. 10.1126/science.6828852.View ArticleGoogle Scholar
- Apfeld J, O'Connor G, McDonagh T, DiStefano PS, Curtis R: The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans. Genes Dev. 2004, 18 (24): 3004-3009. 10.1101/gad.1255404.PubMed CentralView ArticlePubMedGoogle Scholar
- Takeuchi Y, Kishimoto U: Changes of adenine nucleotide levels in Chara internodes during metabolic inhibition. Plant and Cell Physiology. 1983, 24 (8): 1401-1409. [http://pcp.oxfordjournals.org/cgi/content/abstract/24/8/1401]Google Scholar
- Tiwari BS, Belenghi B, Levine A: Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. Plant Physiol. 2002, 128 (4): 1271-1281. 10.1104/pp.010999.PubMed CentralView ArticlePubMedGoogle Scholar
- Gasbarrini A, Borle AB, Farghali H, Bender C, Francavilla A, Van Thiel D: Effect of anoxia on intracellular ATP, Na+i, Ca2+i, Mg2+i, and cytotoxicity in rat hepatocytes. J Biol Chem. 1992, 267 (10): 6654-6663.PubMedGoogle Scholar
- Houthoofd K, Braeckman BP, Lenaerts I, Brys K, De Vreese A, Van Eygen S, Vanfleteren JR: No reduction of metabolic rate in food restricted Caenorhabditis elegans. Exp Gerontol. 2002, 37 (12): 1359-1369. 10.1016/S0531-5565(02)00172-9.View ArticlePubMedGoogle Scholar
- Zhang S, Haldenwang WG: Contributions of ATP, GTP, and redox state to nutritional stress activation of the Bacillus subtilis sigmaB transcription factor. J Bacteriol. 2005, 187 (22): 7554-7560. 10.1128/JB.187.22.7554-7560.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Corton JM, Gillespie JG, Hardie DG: Role of the AMP-activated protein kinase in the cellular stress response. Curr Biol. 1994, 4 (4): 315-324. 10.1016/S0960-9822(00)00070-1.View ArticlePubMedGoogle Scholar
- de Wet JR, Wood KV, DeLuca M, Helinski DR, Subramani S: Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol. 1987, 7 (2): 725-737.PubMed CentralView ArticlePubMedGoogle Scholar
- Lagido C, Pettitt J, Porter AJ, Paton GI, Glover LA: Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEBS Lett. 2001, 493 (1): 36-39. 10.1016/S0014-5793(01)02271-2.View ArticlePubMedGoogle Scholar
- Maechler P, Wang H, Wollheim CB: Continuous monitoring of ATP levels in living insulin secreting cells expressing cytosolic firefly luciferase. FEBS Lett. 1998, 422 (3): 328-332. 10.1016/S0014-5793(97)01618-9.View ArticlePubMedGoogle Scholar
- Schneider DA, Gourse RL: Relationship between growth rate and ATP concentration in Escherichia coli: a bioassay for available cellular ATP. J Biol Chem. 2004, 279 (9): 8262-8268. 10.1074/jbc.M311996200.View ArticlePubMedGoogle Scholar
- Culetto E, Sattelle DB: A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes. Hum Mol Genet. 2000, 9 (6): 869-877. 10.1093/hmg/9.6.869.View ArticlePubMedGoogle Scholar
- Braeckman BP, Houthoofd K, Brys K, Lenaerts I, De Vreese A, Van Eygen S, Raes H, Vanfleteren JR: No reduction of energy metabolism in Clk mutants. Mech Ageing Dev. 2002, 123 (11): 1447-1456. 10.1016/S0047-6374(02)00085-4.View ArticlePubMedGoogle Scholar
- Hewitt EJ, Nicholas DJD: Cations and anions: inhibitors and interactions in metabolism and in enzyme activity. Metabolic inhibitors. Edited by: Hochster RM, Quastel JH. 1963, London , Academic Press, 311-436.View ArticleGoogle Scholar
- Massie MR, Lapoczka EM, Boggs KD, Stine KE, White GE: Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditis elegans. Cell Stress Chaperones. 2003, 8 (1): 1-7. 10.1379/1466-1268(2003)8<1:ETTMIS>2.0.CO;2.PubMed CentralView ArticlePubMedGoogle Scholar
- Jones D, Candido EP: Feeding is inhibited by sublethal concentrations of toxicants and by heat stress in the nematode Caenorhabditis elegans: relationship to the cellular stress response. J Exp Zool. 1999, 284 (2): 147-157. 10.1002/(SICI)1097-010X(19990701)284:2<147::AID-JEZ4>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
- Thompson JF, Hayes LS, Lloyd DB: Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene. 1991, 103 (2): 171-177. 10.1016/0378-1119(91)90270-L.View ArticlePubMedGoogle Scholar
- Collaco AM, Geusz ME: Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice. BMC Physiol. 2003, 3: 8-10.1186/1472-6793-3-8.PubMed CentralView ArticlePubMedGoogle Scholar
- Brandes C, Plautz JD, Stanewsky R, Jamison CF, Straume M, Wood KV, Kay SA, Hall JC: Novel features of Drosophila period transcription revealed by real-time luciferase reporting. Neuron. 1996, 16 (4): 687-692. 10.1016/S0896-6273(00)80088-4.View ArticlePubMedGoogle Scholar
- Mayerhofer R, Araki K, Szalay AA: Monitoring of spatial expression of firefly luciferase in transformed zebrafish. J Biolumin Chemilumin. 1995, 10 (5): 271-275. 10.1002/bio.1170100503.View ArticlePubMedGoogle Scholar
- Millar AJ, Short SR, Chua NH, Kay SA: A novel circadian phenotype based on firefly luciferase expression in transgenic plants. Plant Cell. 1992, 4 (9): 1075-1087. 10.1105/tpc.4.9.1075.PubMed CentralView ArticlePubMedGoogle Scholar
- Keller GA, Gould S, Deluca M, Subramani S: Firefly luciferase is targeted to peroxisomes in mammalian cells. Proc Natl Acad Sci U S A. 1987, 84 (10): 3264-3268. 10.1073/pnas.84.10.3264.PubMed CentralView ArticlePubMedGoogle Scholar
- Nguyen VT, Morange M, Bensaude O: Protein denaturation during heat shock and related stress. Escherichia coli beta-galactosidase and Photinus pyralis luciferase inactivation in mouse cells. J Biol Chem. 1989, 264 (18): 10487-10492.PubMedGoogle Scholar
- Kogai T, Kanamoto Y, Brent GA: The modified firefly luciferase reporter gene (luc+) but not Renilla luciferase is induced by all-trans retinoic acid in MCF-7 breast cancer cells. Breast Cancer Res Treat. 2003, 78 (1): 119-126. 10.1023/A:1022179717847.View ArticlePubMedGoogle Scholar
- Corish P, Tyler-Smith C: Attenuation of green fluorescent protein half-life in mammalian cells. Protein Eng. 1999, 12 (12): 1035-1040. 10.1093/protein/12.12.1035.View ArticlePubMedGoogle Scholar
- Gu T, Orita S, Han M: Caenorhabditis elegans SUR-5, a novel but conserved protein, negatively regulates LET-60 Ras activity during vulval induction. Mol Cell Biol. 1998, 18 (8): 4556-4564.PubMed CentralView ArticlePubMedGoogle Scholar
- Mello C, Fire A: DNA transformation. Methods in Cell Biology. Edited by: Epstein HF, Shakes DC. 1995, San Diego, Academic Press, 48: 451-482.Google Scholar
- Anderson P: Mutagenesis. Methods in Cell Biology. 1995, San Diego , Academic Press, 48: 31-58.Google Scholar
- Lewis JA, Fleming JT: Basic culture methods. Methods in Cell Biology. Edited by: Epstein HF, Shakes DC. 1995, San Diego, Academic Press, 48: 3-29.Google Scholar
- Mörck C, Rauthan M, Wagberg F, Pilon M: pha-2 encodes the C. elegans ortholog of the homeodomain protein HEX and is required for the formation of the pharyngeal isthmus. Dev Biol. 2004, 272 (2): 403-418. 10.1016/j.ydbio.2004.05.011.View ArticlePubMedGoogle Scholar
- Ahringer (ed.) J: Reverse genetics. WormBook. Edited by: WormBook. The C. elegans Research Community, 10.1895/wormbook.1.47.1. [http://www.wormbook.org/chapters/www_introreversegenetics/introreversegenetics.pdf]
- Sokal RR, Rohlf FJ: Biometry: the principles and practice of statistics in biological research. 1995, New York , W. H. Freeman and Company, 887-3rdGoogle Scholar