Figure 8
Treatment of rats with IFN-γ enhances the decrease of the PECAM-1 specific transcript level in CCl 4 -treated rat livers in parallel to an enhanced increase of the ICAM-1 specific transcript level. Animals were treated with CCl4 (-) or with CCl4 and IFN-γ (+) and livers were taken for analysis 3 h (3 h), 6 h (6 h) or 12 h (12 h) after treatment (each time four animals). (c) The untreated control. 10 μg of total RNA was separated on agarose gel, blotted and hybridised with a 32P-labeled cDNA probe specific for rat PECAM-1. The same membranes were hybridised with a cDNA specific for rat ICAM-1 or for β- and γ-actin. This figure shows the results for four experiments. The lower panel shows a statistical evaluation of densitometric scans. Error bars indicate standard deviation of the mean value normalized on β,γ-actin expression of five experiments from five different isolations of each cell type. P-value was tested to show significant differences of rats 12 h after treatment with CCl4 compared to treatment with CCl4 and IFN-γ. * indicates p < 0.05.