Figure 2

Protein expression of AaegPTEN6 in female tissues during a reproductive cycle: To assess the protein expression pattern during reproduction, tissue samples from fully engorged females were collected at various time points (0 (non-fed), 3, 6, 12, 24, 36, 48, and 72 hr (post oviposition)). Tissues from 10–15 females were dissected into 10× PI buffer, size fractioned on 12% SDS-PAGE gels, transferred to PVDF membrane and probed with anti-AaegPTEN 6 antibody. A. AaegPTEN6 was expressed at constant levels throughout the reproductive cycle in heads (HD), midguts (MG) and fat bodies (FB). However, in the ovaries (OV), AaegPTEN6 was strongly expressed prior to and during the first 12 h post-bloodmeal. It could not be detected 24 to 36 h after the bloodmeal, but was again detected at 72 h post-bloodmeal, following oviposition. B. AaegPTEN3 protein in the head was evenly expressed throughout the reproductive cycle. Low expression at 6 and 48 h is due to loading errors as determined by GAPDH expression (not shown).