Impact of gasoline inhalation on some neurobehavioural characteristics of male rats
© Kinawy; licensee BioMed Central Ltd. 2009
Received: 4 March 2009
Accepted: 24 November 2009
Published: 24 November 2009
This paper examines closely and compares the potential hazards of inhalation of two types of gasoline (car fuel). The first type is the commonly use leaded gasoline and the second is the unleaded type enriched with oxygenate additives as lead substituent in order to raise the octane number. The impacts of gasoline exposure on Na+, K+-ATPase, superoxide dismutase (SOD), acetylcholinesterase (AChE), total protein, reduced glutathione (GSH), and lipid peroxidation (TBARS) in the cerebral cortex, and monoamine neurotransmitters dopamine (DA), norepinephrine (NE) and serotonin (5-HT) in the cerebral cortex, hippocampus, cerebellum and hypothalamus were evaluated. The effect of gasoline exposure on the aggressive behaviour tests was also studied.
The present results revealed that gasoline inhalation induced significant fluctuations in the levels of the monoamine neurotransmitters in the studied brain regions. This was concomitant with a decrease in Na+, K+-ATPase activity and total protein content. Moreover, the group exposed to the unleaded gasoline exhibited an increase in lipid peroxidation and a decrease in AChE and superoxide dismutase activities. These physiological impairments were accompanied with a higher tendency towards aggressive behaviour as a consequence to gasoline inhalation.
It is concluded from the present work that chronic exposure to either the leaded or the unleaded gasoline vapours impaired the levels of monoamine neurotransmitters and other biochemical parameters in different brain areas and modulated several behavioural aspects related to aggression in rats.
Gasoline is the generic term for petroleum fuel used mainly for internal combustion engines. It is complex, volatile, and highly flammable and contains over 500 saturated or unsaturated hydrocarbons having from 3 to 12 carbon atoms. About 110 million people are exposed to gasoline constituents in the course of refueling at gasoline stations . Major toxic risk comes from breathing exhaust fumes and evaporative and refueling emissions rather than from occasional skin contact from spills . Nonetheless, after gasoline application to skin, a decrease in glutathione concentration, glutathione S -transferase activity, and lipid peroxidation was observed in liver and brain . Gasoline-induced neurotoxic effects such as ataxia, tremor, acute or subacute encephalopathic syndrome were ascribable to intentional use (gasoline sniffing) and not to occupational exposure. Unfortunately, gasoline sniffing has become an increasingly rising phenomenon in the poor societies as a means for cheap mood alteration .
Leaded gasoline contains a range of organolead compounds, especially tetraethyl lead, are difficult to eliminate from the CNS, and the injuries induced usually result in permanent neurological deficits that cause medical as well as socio-economical problems . Ethylene glycol is another gasoline constituent and a general fuel additive widely used in automobiles for its ability to absorb water and to prevent overheating or freezing. Ingestion of ethylene glycol produces not only central nervous system depression, but also cardiopulmonary complications, acute renal failure, and delayed neurological sequelae .
The change from leaded to unleaded gasoline, in fact, represents a transformation of a risk factor for the human population chronically exposed to urban air polluted by automobile smokes .
Oxygenates include substances such as methanol, ethanol, Methyl tertiary butyl ether (MTBE), ethyl tertiary butyl ether (ETBE), tertiary butyl alcohol (TBA), and tertiary amyl methyl ether (TAME) are used as antiknock agents in place of lead derivatives and as substitutes for high octane aromatics in fuel [8, 9]. Methanol are toxic alcohols can be used as a fuel either alone or as a gasoline additive. Methanol is well absorbed in humans following inhalation, ingestion, or cutaneous exposure; it produces a transient mild depression of the CNS with headache, vertigo, and vomiting. Ethanol induced an increase in the levels of norepinephrine in paternally exposed offspring. Serotonin levels were reduced in the cerebrum, and met-enkephalin levels were increased in all brain regions of offspring from both maternally and paternally exposed rats . Methyl tertiary butyl ether (MTBE) is an aliphatic ether, a volatile, colorless, and inflammable liquid, currently the most widely used oxygenate as an octane enhancer in gasoline blends. In subchronic and chronic toxicity studies, MTBE appears to have low systemic toxicity; the main findings observed in rats were depressant effects on the CNS, typical of similar ethers [11, 12]. Tertiary amyl methyl ether (TAME) causes a significant but transient CNS depression akin to, but slightly more severe than, that resulting from MTBE exposure at the same levels . Methylcyclopentadienyl manganese tricarbonyl (MMT) is an effective octane enhancer. The primary combustion products of MMT are Mn phosphate, Mn sulfate, and a Mn phosphate/Mn sulfate mixture. Concerns have been raised that the combustion products of MMT containing Mn could cause neurological symptoms similar to Parkinson's disease in humans, even at low levels of exposure [14, 15]. In rats, increase in brain Mn delivery was observed with inhalation exposure following Mn absorption from the pulmonary tract and direct transport of Mn to the central nervous system along the olfactory nerve . High dose manganese selectively targets dopaminergic neurons in the human basal ganglia [17, 18]. Another study by Erikson et al  found that rats had significantly decreased striatal glutathione (GSH) levels following subchronic MnSO4 inhalation.
Alkylbenzenes, including toluene and xylene, occur naturally in small amounts in gasoline blends and standard gasoline formulations. These compounds are mainly absorbed by inhalation causing dysfunction in the CNS. The effects vary from severe neurological disorder (acute inhalation due to sniffing abuse) to deficits in neurobehavioural function in occupationally exposed groups. Neurobehavioural effects deriving from sub-acute exposure to toluene have been investigated in rats  and mice .
As Egypt is now in the process of transferring into the use of unleaded gasoline, this study employed the two types of gasoline currently used in Egypt and focused on comparing their impact. The study was designed to evaluate the effects of gasoline vapour inhalation on the brain monoamine neurotransmitters, lipid peroxidation, GSH, SOD, Na+, K+-ATPase and AChE. In addition to these biochemical analyses, the impact of gasoline inhalation on the aggressive behaviour was assessed.
Male Wister rats weighing (130-160 g) obtained from the institute of Ophthalmic Disease Research were used and housed two per cage. Animals were kept in a controlled environment of 12 h light- dark cycle and 22 ± 2°c room temperature. Food and water were provided ad libitum. Animals were maintained at the Faculty of Science, Ain Shams University animal unit where housing, handling, and use of animals were strictly in agreement with the regulations and guidelines set by the Faculty's research ethics committee.
Gasoline vapour inhalation studies
The dynamic system of exposure to gasoline vapours which closely matches exposure to vapour in open air was applied in this study. The dynamic model of exposure to vapours is preferred to the static system as the latter allows the concentration of gas to build up and increase as time goes by Haschek et al .
The LC 50 (30 minutes) for leaded (G1) was found to be 37475 ppm and for unleaded (G2) 39928 ppm.
The rats of each group were subjected to aggressive behaviour tests for ten days, through the last ten days of exposure. After the completion of this test, the 15 animals of each group were decapitated and the brain was quickly removed out from each rat. The cerebral cortex was excised, from the level of the pituitary gland to the end of the olfactory lobe, for the determination of lipid peroxidation, GSH, SOD, protein, Na+, K+-ATPase, the AChE and monoamines. Hippocampus, hypothalamus and cerebellum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.
A- Aggressive behaviour evaluations
The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The following parameters were determined according to Barnett :
▪ Tooth chattering: it goes on while the rat remains immobile, after he has detected the presence of an opponent, and while he approaches the latter.
▪ Threat posture: the back is maximally arched by the attacker, all fore limbs are extended and the flank is turned towards the opponent.
▪ Leaping and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at him by rapidly adducting his extended fore limbs and he also bites the ear or limb or the tail.
▪ Boxing position: longer intervals in which both rats adopt a defensive attitude.
B- Physiological and biochemical measurements
Determination of superoxide dismutase (SOD) activity
Superoxide dismutase (SOD) activity was assayed by the procedure of Niskikimi et al. . The assay relies on the ability of the enzyme to inhibit phenazine methosulphate-mediated reaction of nitroblue tetrazolium dye.
Determination of reduced glutathione (GSH) content
This method was described by Prins and Loose  and depends on the precipitation of protein using tungstate-sulfuric acid solution and the formation of a yellow colour after reacting with 5,5'dithiobis-2-nitrobenzoic acid (DTNB) which is then read at 412 nm.
Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate
This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm .
HPLC determination of the brain monoamine concentrations
Preparation of the sample
The first step in determination of the brain monoamine by HPLC method involved weighing and homogenization of the tissue in 1/10 weight/volume of 75% aqueous HPLC grade methanol. The homogenate was spun at 3000 r.p.m. for 10 min and the supernatant was used for monoamine determination immediately extracted from the trace elements and lipids by the use of solid phase extraction CHROMABOND column NH2 phase Cat. No. 730031. The sample was then injected directly into an AQUA column 150 54.6 mm 5 μC18, purchased from Phenomenex, USA under the following conditions: mobile phase 97/3 20 Mm potassium phosphate, pH 3.0/methanol, flow rate 1.5 ml/min, UV 270 nm. norepinephrine, dopamine and serotonin were separated after 12 minutes. The resulting chromatogram identified each monoamine position and concentration from the sample as compared to that of the standard, and finally, the determination of the content of each monoamine as μg per gram brain tissue .
Determination of acetylcholinesterase (AChE) activity
The AChE activity was estimated using Ellman procedure  as modified by Gorun et al.  for the assay of purified preparations of cholinesterases. This procedure is based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2-nitrobenzoic acid (DTNB). However, the original Ellman procedure was found inadequate for crude enzymatic preparations due to the interfering reactions of free SH groups of protein with DTNB. These limitations have been overcome by transforming the kinetic Ellman procedure into static one where the cholinesterases were incubated with an acylthiocholine for a specific time interval and the reaction was then stopped and the colour was developed upon the addition of DTNB - Phosphate - ethanol reagent. The colour was then read immediately at 412 nm and the enzyme activity was determined in μM per SH group (μMSH) from a standard curve.
Determination of total proteins
The total protein content was determined according to the method described by Lowry et al. . This method is based on the reaction between the tryptophan and tyrosine residues of the protein and the phosphomolybdic - phosphotungestic acid reagent (commercially known as Folin Ciocalteu's reagent) in alkaline copper solution. The developed blue colour was then measured colouremetrically at a wave-length of 690 nm.
Determination of total adenosine triphosphatase (Na+, K+-ATPase) activity
The principle of the assay depends on the incubation of adenosine triphosphate with the tissue-containing enzyme and the determination of liberated inorganic phosphorus as modified by El-Aaser and El-Merzabani .
The data were subjected to statistical analysis using one way ANOVA. SPSS for Windows software, Release 11.0 (SPSS, Chicago, IL) was used.
Effect of chronic exposure to two types of gasoline vapour on the content of monoamines (μg/g) in the cerebral cortex of male rats.
0.496 ± 0.045
1.3 ± 0.11
0.46 ± 0.06
0.488 ± 0.054
1.36 ± 0.35
0.47 ± 0.18
0.428 ± 0.062 a*
0.95 ± 0.06 a* b*
0.498 ± 0.053
Effect of chronic exposure to two types of gasoline vapour on the content of monoamines (μg/g) in the hypothalamus of male rats
0.115 ± 0.031
2.68 ± 0.27
0.822 ± 0.047
0.11 ± 0.013
1.136 ± 0.29 a***
0.50 ± 0.075 a***
0.076 ± 0.0038 a** b**
1.61 ± 0.28 a*** b*
0.49 ± 0.13 a***
Effect of chronic exposure to two types of gasoline vapour on the content of monoamines (μg/g) in the hippocampus of male rats
0.54 ± 0.066
1.82 ± 0.125
0.65 ± 0.12
0.23 ± 0.035 a***
1.53 ± 0.29 a*
0.4 ± 0.15 a**
0.25 ± 0.12 a***
1.02 ± 0.11 a*** b**
0.39 ± 0.077 a**
Effect of chronic exposure to two types of gasoline vapour on the content of monoamines (μg/g) in the cerebellum of male rats
0.376 ± 0.07
1.45 ± 0.12
0.80 ± 0.115
0.47 ± 0.086 a*
1.71 ± 0.14 a*
0.60 ± 0.043 a**
0.41 ± 0.036
1.35 ± 0.24 b**
0.385 ± 0.057 a*** b**
Effect of chronic exposure to two types of gasoline vapour on the acetylcholinesterase (AChE) activity, Na+, K+-ATPase activity and total protein of cerebral cortex in male rats
μmol/pi-min/g wet tissue
g/100 g tissue
17.5 ± 2.24
16.34 ± 1.51
6.63 ± 0.26
16.25 ± 2.2
13.5 ± 1.63 a*
5.78 ± 0.39a**
12.5 ± 2.62 a** b*
14.21 ± 1.92 a*
6.05 ± 0.38 a*
Effect of chronic exposure to two types of gasoline vapour on the contents of lipid peroxidation, reduced glutathione and superoxide dismutase activity of cerebral cortex in male rats.
4.35 ± 0.79
4.456 ± 0.71
6.57 ± 1.37 a** b**
mgGSH/g wet tissue
5.834 ± 0.76
3.635 ± 0.43 a***
4.7 ± 0.86 a* b*
Unit/g wet tissue
267.26 ± 18.46
253.79 ± 14.74
192.56 ± 19.78 a*** b***
Effect of chronic exposure to two types of gasoline vapour on the number of aggressive behaviour in male rats.
Number of aggression events
Leaping and biting
1.2 ± 0.33
1.1 ± 0.31
0.9 ± 0.35
0.8 ± 0.25
3.7 ± 0.63 a*
3.9 ± 0.43 a**
3.7 ± 0.58 a**
2.7 ± 0.47 a*
4 ± 0.87 a*
3.6 ± 0.87 a*
3.4 ± 0.70 a*
3.2 ± 0.74 a**
Effect of chronic exposure to two types of gasoline vapour on the duration of aggressive behaviour in male rats.
Duration of aggression/sec
Leaping and biting
0.9 ± 0.23
1.5 ± 0.54
1.9 ± 0.72
1 ± 0.42
4.7 ± 1.1 a**
6 ± 1.2 a*
5.5 ± 1.45
2.2 ± 0.63
4.4 ± 0.92 a*
6.2 ± 1.31 a*
5.9 ± 1.50
2.2 ± 0.74
The present study revealed that rats exposed to the unleaded-gasoline had significantly elevated levels of lipid peroxides and a decrease in the superoxide dismustase activity in the brain cortex region in comparison with the control or with the group exposed to the leaded gasoline. Lipid peroxidation is a marker of oxidative stress that facilitates the evaluation of the damage caused by free radicals on membrane lipids [34, 35]. On the other hand, GSH was lower in either the leaded gasoline or the unleaded gasoline than the control group. Correlative study by Raza  revealed that after gasoline application to skin, a decrease in glutathione concentration, glutathione S -transferase activity, and lipid peroxidation was observed in liver and brain. Exposure to toluene, one of the gasoline constituents, both in vivo and in vitro, leads to reactive oxygen species formation in many tissues including brain tissue [36–38]. The study of Calderón-Guzmán et al  revealed that the aldehydes resulting from the oxidation of the methyl groups in the aromatic compounds of toluene and cresols play an important role in the cell-damage process due to their lipophilic properties. A similar result indicated that exposure to low concentrations of toluene leads to persistent effects on cognitive, neurological, and brain-structural properties in the rat . On the other hand, the results derived from the present trial indicate that Na+, K+-ATPase activity and total protein content in the leaded or unleaded gasoline groups were lower than those of the control group. The inhibition of the plasma membrane Na+, K+-ATPase might have been attributed to a direct effect of the solvents and alcohols in the gasoline on the membrane causing dissolving the its bilayers. The most outstanding are toluene (highly lipophylic) acts on membrane lipids and the effect is specifically on phospholipids and on the other hand the organic solvents act on membrane proteins [34, 41, 42]. Also as the functional hydroxyl group (OH-) moves away from the functional methyl group (CH3) attached to the aromatic ring of the solvents, the biogenic amines, lipid peroxidation, and Na+, K+-ATPase and total ATPase enzyme activity tend to suffer important changes, indicating that the stearic impediment effect and the inductive effect produced at the aromatic ring of the solvents play an important role in the response of biogenic amine metabolism, together with the enzymes and lipid peroxidation of animal brain membranes . Other study by Castilla et al  reported a reduction of the enzyme Na+, K+-ATPase in brain membranes when high doses of toluene vapour (30,000 ppm) were administered subchronically to rats, but there was an increased Na+, K+-ATPase activity during acute exposure. In agreement of this line of reasoning is the study of Gutteridge and Halliwell  showing that Na+, K+-ATPase reduces its activity up to 50% due to oxidative stress. The present findings show that the rats exhibited a decrease in the activity of AChE in the unleaded group in comparison with either the control or the leaded groups. In addition to its function in degrading acetylcholine and modulation of neural function; AChE as a structural protein appears to play an important role in axonal outgrowth . Correlative study by Capo et al  on ethylene glycol, which is consider one of the main constitutes of gasoline, demonstrated various types of neuronal toxicities after ethylene glycol intoxication. The mechanism could possibly be related to oxidation of cellular macromolecules or to relative reduction in acetylcholinesterase-containing neurons, thus potentiating cholinergic effects. In the present study, no statistical differences were detected among the serotonin levels of the cerebral cortex in the three groups; the control, the leaded and the unleaded. However, in the hypothalamus, hippocampus, cerebellum, the serotonin level was lower in the groups exposed to either type of gasoline as compared to the control group. The serotonin system originates in Raphe nucleus in brain stem and projects with extensive connections to the hypothalamus, cerebellum, and cereberum  and plays an important role in regulation of behaviour, mood, appetite, anxiety and sleep . The rats exposed to unleaded gasoline had a significant decrease in the dopamine of the cerebral cortex as compared with the other two groups, but in the hypothalamus and hippocampus dopamine was lower in either the leaded or unleaded group as compared with the control. On the contrary, dopamine was elevated in the cerebellum of leaded exposed group above both the unleaded gasoline and control. The fluctuations in the levels of dopamine in different brain areas may be related to the effects MMT which is an organic manganese (Mn) compound added to unleaded gasoline and the mechanisms of Mn neurotoxicity has focused on the oxidative properties of Mn and its interactions with the dopaminergic system, including biphasic increases and decreases in dopamine levels associated with Mn exposure . Also, Prabhakaran et al  suggested a new underlying mechanism in which Mn interaction with cellular DA to potentiate ROS activates NF-B as well as iNOS signaling, and all of which are involved in promoting dopaminergic cell death. On the other hand, another gasoline constituent; ethylene glycol was found to target the dopaminergic neurons in the basal ganglia thereby inducing serious intoxication manifested as focal inflammation and necrosis in the basal ganglia areas of the brain and these effects are associated with Parkinsonism . Toluene, another gasoline constituent, was reported to induce perturbation in the nigrostriatal tract responsible for the coordination of voluntary movement . The cell bodies of the dopamine system are located in the substabtia nigra of midbrain and project to the striatum of the basal ganglia to coordinate skeletal muscle contraction, moreover, DA innervations reaches out to the hypothalamus to regulate secretion of TRH and prolactin secretion, and then projects to the brain limbic system to modulate motivations and emotions . So, gasoline induced impairment of the DA system would result in serious impacts on the neural control of voluntary locomotion and would affect several behavioural aspects.
In the present study, the norepinephrine level in the cerebral cortex and hypothalamus of the unleaded group was less than that in the control. Also norepinephrine was reduced in the hippocampus of both unleaded and leaded gasoline groups in comparison with the control. In the cerebellum, norepinephrine level was higher in the leaded group as compared with the control group. It was reported that volatile solvents such as benzene, a main constituent of gasoline fuel, seems to interact with the synthesis and catabolism of catecholamines and serotonin in the brain, which might explain the neurotoxic effects of these solvents . In this regard, deficiencies of serotonin or other monoamine neurotransmitters such as dopamine and norepinephrine are linked with depression [54, 55]. In reference to the present results of the behavioural tests, the rats exposed to either the unleaded or the leaded gasoline exhibited a significantly higher rate of anxiety and emotionality in comparison with the control animals. Although information related to the effect of gasoline inhalation on behavioural aspects is extremely scarce, yet, it is possible to interpret these results in the light of the effects of gasoline constituents. Another study demonstrated that lead exposure enhances predatory aggression in the cat and provide experimental support for a causal relationship between lead exposure and aggressive behaviour in humans . This was concomitant with deficiency in serotonin that plays an important role to counteract the aggressive behaviour [57, 58]. A correlative study by Perreault et al  found that aggressive male coral reef fish had lower relative serotonergic activity than less aggressive males, and treatment with the serotonin reuptake inhibitor antidepressant drug flouxotine reduce the territorial aggressive behaviour. It was postulated that serotonin helps counterbalance the tendency of two other major neurotransmitters in the brain dopamine and noradrenaline to encourage overarousal, fear, anger, tension, aggression, violence, obsessive-compulsive actions, overeating, anxiety and sleep disturbances .
The present results revealed that gasoline inhalation induced significant fluctuations in the levels of the monoamine neurotransmitters in the cerebral cortex, hypothalamus, hippocampus and cerebellum. The group exposed to the unleaded gasoline exhibited an increase in lipid peroxidation and a decrease in AChE and superoxide dismutase. The physiological impairments were accompanied with a higher tendency towards aggressive behaviour as a consequence to gasoline inhalation.
K+-ATPase: total adenosine triphosphatase
central nervous system
reactive oxygen species
methylcyclopentadienyl manganese tricarbonyl
Tertiary amyl methyl ether
Methyl tertiary butyl ether
ethyl tertiary butyl ether
tertiary butyl alcohol.
I wish to express my sincere appreciation to Dr. Ahmed R. Ezzat, Professor of Physiology, Faculty of Science, Ain Shams University for his kind support and guidance throughout this work and for providing the necessary funding and laboratory facilities.
- Wixtrom RN, Brown SL: Individual and population exposures to gasoline. J Expo Anal Environ Epidemiol. 1992, 2: 23-78.PubMed
- Dutch Expert Committee for Occupational Standards (DECOS): Health Based Recommended Occupational Exposure Limit for Gasoline. 1992, RA 3/92. Den Haag, Netherlands: Directorate General of Labour
- Raza H, Qureshi MM, Montague W: Alteration of glutathione, glutathione S-transferase and lipid peroxidation in mouse skin and extracutaneous tissues after topical application of gasoline. Int J Biochem Cell Biol. 1995, 27: 271-277. 10.1016/1357-2725(95)93663-I.View ArticlePubMed
- Burbacher TM: Neurotoxic effects of gasoline and gasoline constituents. Environ Health Perspect. 1993, 101 (suppl 6): 133-141.PubMed CentralView ArticlePubMed
- Chang LW: The neurotoxicology and pathology of organomercury, organolead, and organotin. J Toxicol Sci. 1990, 15: S125-S151.View Article
- Lewis LD, Smith BW, Mamourian AC: Delayed sequelae after acute overdoses or poisonings: cranial neuropathy related to ethylene glycol ingestion. Clin Pharm Ther. 1997, 61: 692-699. 10.1016/S0009-9236(97)90105-3.View Article
- Gilli G, Scursatone E, Bono R: Geographical distribution of benzene in air in north-western Italy and personal exposure. Environmental Health Perspectives. 1996, 104 (Suppl 6): 1137-1140. 10.2307/3433153.PubMed CentralView ArticlePubMed
- Schuetzle D, Siegl WO, Jensen TE, Dearth MA, Kaiser EW, Gorse R, Kreucher W, Kulik E: The relationship between gasoline composition and vehicle hydrocarbon emissions: a review of current studies and future research needs. Environ Health Perspect. 1994, 102 (suppl 4): 3-12. 10.2307/3431925.PubMed CentralView ArticlePubMed
- Born GL, Lucas SV, Scott RD, DeFries TH, Kishan S: Effect of use of low oxygenate gasoline blends upon emissions from California vehicles. 1994, Govt Reports Announcements & Index (GRA&I), 23
- Nelson BK, Brightwell WS, MacKenzie-Taylor DR, Burg JR, Massari VJ: Neurochemical, but not behavioral, deviations in the offspring of rats following prenatal or paternal inhalation exposure to ethanol. Neurotoxicology and Teratology. 1988, 10 (1): 15-22. 10.1016/0892-0362(88)90062-1.View ArticlePubMed
- Daughtrey WC, Gill MW, Pritts IM, Douglas JF, Kneiss JJ, Andrews LS: Neurotoxicological evaluation of methyl tertiary-butyl ether in rats. J Appl Toxicol. 1997, 17: S57-64. 10.1002/(SICI)1099-1263(199705)17:1+<S57::AID-JAT411>3.3.CO;2-1.View ArticlePubMed
- Lington AW, Dodd DE, Ridlon SA, Douglas JF, Kneiss JJ, Andrews LS: Evaluation of 13-week inhalation toxicity study on methyl t-butyl ether (MTBE) in Fisher 344 rats. J Appl Toxicol. 1997, 17: S37-44. 10.1002/(SICI)1099-1263(199705)17:1+<S37::AID-JAT409>3.3.CO;2-H.View ArticlePubMed
- White RD, Daughtrey WC, Wells MS: Health effects of inhaled tertiary amyl methyl ether and ethyl. Toxicol Lett. 1995, 82: 719-724. 10.1016/0378-4274(95)03590-7.View ArticlePubMed
- Normandin L, Carrier G, Gardiner PF, Kennedy G, Hazell AS, Mergler D, Butterworth RF, Philippe S, Zayed J: Assessment of Bioaccumulation, Neuropathology, and Neurobehavior Following Subchronic (90 Days) Inhalation in Sprague-Dawley Rats Exposed to Manganese Phosphate. Toxicology and Applied pharmacology. 2002, 138: 135-145. 10.1016/S0041-008X(02)99464-0.View Article
- Salehi F, Krewski D, Mergler D, Normandin L, Kennedy G, Philippe S, Zayed J: Bioaccumulation and locomotor effects of manganese phosphate/sulfate mixture in Sprague-Dawley rats following subchronic (90 days) inhalation exposure. Toxicology and Applied Pharmacology. 2003, 191 (3): 264-271. 10.1016/S0041-008X(03)00238-2.View ArticlePubMed
- Brenneman KA, Cattly R, Ali S, Dorman D: Manganese-induced developmental neurotoxicity in the CD rat: is oxidative damage a mechanism of action. Neurotoxicology. 1999, 20: 477-488.PubMed
- Pal PK, Samii A, Calne DB: Manganese neurotoxicity: a review of clinical features, imaging and pathology. Neurotoxicology. 1999, 20: 227-238.PubMed
- Olanow CW: Manganese-induced Parkinsonism and Parkinson's disease. Ann N Y Acad Sci. 2004, 1012: 209-223. 10.1196/annals.1306.018.View ArticlePubMed
- Erikson KM, Dorman DC, Lash LH, Dobson AW, Aschner M: Airborne manganese exposure differentially affects end points of oxidative stress in an age- and sex-dependent manner. Biol Trace Elem Res. 2004, 100: 49-62. 10.1385/BTER:100:1:049.View ArticlePubMed
- Saito K, Wada H: Behavioral approaches to toluene intoxication. Environ Res. 1993, 62: 53-62. 10.1006/enrs.1993.1088.View ArticlePubMed
- Tegeris JS, Balster RLA: Comparison of the acute behavioral effects of alkylbenzenes using a functional observational battery in mice. Fundam Appl Toxicol. 1994, 22: 240-250. 10.1006/faat.1994.1028.View ArticlePubMed
- Haschek WM, Witschi HR: Respiratory system. Handbook of Toxicologic Pathology. Edited by: Haschek WM, Rousseaux CG. 1991, Academic Press, INC. Harcourt Brace Jovanovich, publisher. San Diego, 78-79.
- Ezzat AR, Rouina I, Fares NH: Effect of chronic exposure to two types of gasoline vapours on the free amino acid profile and structural cerebral cortex in the albino rat. J Egypt Ger Soc Zool. 2001, 34 (A): 255-276.
- Smith JM, Van Ness HC: Introduction to chemistry engineering thermodynamic. 1959, McGraw-Hill Companies, 67-2
- Barnett SA: A study in behaviour. 1967, Camelot Press Ltd., London, 85-89.
- Niskikimi M, Roa NA, Yagi K: The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biophys Res Commun. 1972, 46: 849-854. 10.1016/S0006-291X(72)80218-3.View Article
- Prins GK, Loose JA: Glutathione. Biochemical methods in red cell genetics. 1969, Academic press N. Y. D. London, Chapter 4: 126-129.
- Stroev EA, Makarova VG: Study in peroxidation of biological membrane lipids. 1988, Laboratory manual in biochemistry. English translation, Chapter 15: 251-255.
- Pagel P, Blome J, Wolf HU: High-performance liquid chromatographic separation and measurement of various biogenic compounds possibly involved in the pathomechanism of Parkinson's disease. J Chromatog B. 2000, 746: 297-304. 10.1016/S0378-4347(00)00348-0.View Article
- Ellman GL, Courtney KD, Andres V, Featherstone RM: A new and rapid calorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961, 7: 88-95. 10.1016/0006-2952(61)90145-9.View ArticlePubMed
- Gorun V, Proinov I, Baltescu V, Balaban G, Barzu : Modefied Ellman Procedure for assay of cholinesterase in crude enzymatic preparations. Anal Biochem. 1978, 86: 324-326. 10.1016/0003-2697(78)90350-0.View ArticlePubMed
- Lowry GM, Rosenbrovgh NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J boil Chem. 1951, 193: 265-275.
- El Aaser AA, El Merzabani MM: Simultaneous determination of 5-neucleotidase and alkaline phosphatase activities in serum. Z Klin Chem. 1975, 13: 453-
- Lebel CP, Schatz RA: Altered synaptosomal phospholipid metabolism after toluene: possible relationship with membrane fluidity, Na+, K+-adenosine triphosphatase and phospholipid methylation. J pharmacol Exp Ther. 1990, 253: 1189-1197.PubMed
- Baydas G, Ozveren F, Tuzcu M, Yasar A: Effects of thinner exposure on the expression pattern of neural cell adhesion molecules, level of lipid peroxidation in the brain and cognitive function in rats. Euro J Pharm. 2005, 512 (2-3): 181-187. 10.1016/j.ejphar.2005.02.038.View Article
- Mattia CJ, Ali SF, Bondy SC: Toluene-induced oxidative stress in several brain regions and other organs. Mol Chem Neuropathol. 1993, 3: 313-328. 10.1007/BF03160122.View Article
- Halifeoglu I, Canatan H, Ustundag B, Ilhan N, Inan F: Effect of thinner inhalation on lipid peroxidation and some antioxidant enzymes of people working with paint thinner. Cell Biochem Funct. 2000, 18: 263-267. 10.1002/1099-0844(200012)18:4<263::AID-CBF882>3.0.CO;2-1.View ArticlePubMed
- Burmistrov SO, Arutyunyan AV, Stepanov MG, Oparina TI, Prokopenko VM: Effect of chronic inhalation of toluene and dioxane on activity of free radical processes in rat ovaries and brain. Bull Exp Biol Med. 2001, 3: 832-836. 10.1023/A:1013106315140.View Article
- Calderón-Guzmán D, Hernández-Islas JL, Espítia IR: Effect of toluene and cresols on Na+, K+-ATPase, and serotonin in rat brain. Regulatory Toxicology and Pharmacology. 2005, 41 (1): 1-5. 10.1016/j.yrtph.2004.09.005.View ArticlePubMed
- Euler MV, Pham TM, Hillefors M, Bjelke B, Henriksson B, Euler GV: Inhalation of Low Concentrations of Toluene Induces Persistent Effects on a Learning Retention Task, Beam-Walk Performance, and Cerebrocortical Size in the Rat. Exp Neurol. 2000, 163 (1): 1-8. 10.1006/exnr.1999.7288.View Article
- Korpela M, Tahti H: The effect of in vitro and in vivo toluene exposure on rat erythrocyte and synaptosome membrane integral enzymes. Pharmacol Toxicol. 1988, 63: 30-32. 10.1111/j.1600-0773.1988.tb00904.x.View ArticlePubMed
- Franks NP, Lieb WR: Anaesthetics on the mind. Nature (Lond). 1987, 328: 113-114. 10.1038/328113a0.View Article
- Castilla SL, Gutierrez GA, Labra RN, Calderón GD, Barragán MG, Rodríguez PR: Effects of toluene exposure on ATPase activity in synaptosomal membranes of rat brain in vivo and in vitro studies. Arch Inst Nac Neurol. 1995, 10: 61-67.
- Gutteridge MC, Halliwell B: The Measurement and Mechanism of Lipid Peroxidation in Biological Systems. 1990, Elsevier, Amsterdam
- Bigbee JW, Sharma KV, Chan ELP, Bogler O: Evidence for the direct role of acetylcholinesterase in neurite outgrowth in primary dorsal root ganglion neurons. Brain Res. 2000, 861: 354-362. 10.1016/S0006-8993(00)02046-1.View ArticlePubMed
- Capo MA, Sevil MB, Lopez ME: Ethylene glycol action and its cholinomimetic effects. J Environ Pathol Toxicol Oncol. 1993, 12: 155-159.PubMed
- Waxman S: Correlative Neuroanatomy. 1996, Appelton & Lang Publishers, Norwalk, Connecticut, California, 23
- George J, Siegel MD: Basic neurochemistry: Molecular, Cellular and Medical Aspects. 1999, Williams L and Wilkins, USA, 6
- Heilbronn E, Eriksson H: Implications of manganese in disease, especially central nervous system disorders. In: Mineral and Metal. Neurotoxicology. Edited by: Yasui M, Strong, MJ, Ota K, Verity MA. 1996, Boca Raton, FL: CRC Press, 311-317.
- Prabhakaran K, Ghosh D, Chapman GD, Gunasekar PG: Molecular mechanism of manganese exposure-induced dopaminergic toxicity. Brain Research Bulletin. 2008, 76: 361-367. 10.1016/j.brainresbull.2008.03.004.View ArticlePubMed
- Reddy NJ, Lewis LD, Gardner TB, Osterling W, Eskey CJ, Nierenberg DW: Two cases of rapid onset Parkinson's syndrome following toxic ingestion of ethylene glycol and methanol. Clinical Pharmacology & Therapeutics. 2007, 81: 114-121. 10.1038/sj.clpt.6100013.View Article
- Cintra A, Andbjer B, Finnman UB, Hagman M, Agnati LF, Hoglund G, Fuxe K: Subacute toluene exposure increases DA dysfunction in the 6-OH dopamine lesioned nigrostriatal dopaminergic system of the rat. Neurosci Lett. 1996, 217: 61-65. 10.1016/0304-3940(96)13049-4.View ArticlePubMed
- European Chemicals Bureau: RISK ASSESSMENT Benzene (CAS-No. 71.43.2), draft 2003a. Ispra, Italy. [http://ecb.jrc.ec.europa.eu/]
- Mann JJ: Role of the serotonergic system in the pathogenesis of major depression and suicidal behaviour. Neuropsychopharmacology. 1999, 21: 99S-105S.View ArticlePubMed
- Kinawy AA: Possible ameliorative effects of desmopressin and fluoxtine on cinnarizine- induced disturbance in brain monoamine neurotransmitters and behaviour in male rats. J Egypt Ger Soc Zool. 2007, 49-77. German Egyptian Science week 17th July _1st August 2007 in Dresden - Dusseldorf. Germany, 54 A
- Wenjie LA, Shenggao HA, Thomas R, Gregg b, Francis W, Kemp A, Amy L, Davidow A, Donald B, Louria A, Allan Siegel B, Bogdena JD: Lead exposure potentiates predatory attack behavior in the cat. Environmental Research. 2003, 92: 197-206. 10.1016/S0013-9351(02)00083-X.View Article
- Ferris CF, Delville Y: Vasopressin and serotonin interactions in the control of agonistic behaviour. Psychoneuroendocrinology. 1994, 19: 593-601. 10.1016/0306-4530(94)90043-4.View ArticlePubMed
- Ferris CF, Stolberg T, Delville Y: Serotonin regulation of aggressive behaviour in Male Golden Hamsters (Mesocricetus auratus). Behavioural Neuroscience. 1999, 113 (4): 804-815. 10.1037/0735-7044.113.4.804.View Article
- Perreault HA, Semsar K, Godwin J: Fluoxetine treatment decreases territorial aggression in a coral reef fish. Physiol Behav. 2003, 79 (4-5): 719-24. 10.1016/S0031-9384(03)00211-7.View ArticlePubMed
- Robertson J, Monte T: Natural prozac-learning to release your body's own anti-depressants. 1997, San Francisco: Harper
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