Table 2 Primers sequences used for RT-PCR analysis of mRNA targets
mRNA target mRNA | PCR primer sequence 5’→ 3’ | Genbank |
|---|---|---|
18S RNA | Forward: CGAACGTCTGCCCTATCAACTT | EU 139318.1 |
Reverse: ACCCGTGGTCACCATGGTA | ||
COXI | Forward: TGCCAGTATTAGCAGCAGGT | X14848.1 |
Reverse: GAATTGGGTCTCCACCTCCA | ||
COXIV | Forward: AGTCCAATTGTACCGCATCC | NM 017202.1 |
Reverse: ACTCATTGGTGCCCTTGTTC | ||
SDHB | Forward: CAGAGAAGGGATCTGTGGCT | NM 001100539.1 |
Reverse: TGTTGCCTCCGTTGATGTTC | ||
PGC-1α | Forward: ATGAGAAGCGGGAGTCTGAA | NM 031347.1 |
Reverse: GCGCTCTTCAATTGCTTTCT | ||
SERCA2A | Forward: GGCCCGAAACTACCTGGAGCC | NM 001110139 |
Reverse: CAACGCACATGCACGCACCC | ||
CAV3 | Forward: CCCAAGAACATCAATGAGGAC | U31968 |
Reverse: GGAGACGGTGAAAGTGGTGT | ||
DYSF | Forward: TGGGAACCGCTACCATCTAC | NM001107869 |
Reverse: CTCTGGTGCAGGAAGGAGAC |