Fig. 1

Western blot analysis of hybridoma immune-reactivity MBP and GST tagged recombinant mouse PTMA (as shown in the inlet) were loaded onto gel for electrophoresis and thereafter blotted on to nitrocellulose membranes. Blotted membranes were incubated with supernatant from the hybridoma cultures from different clones as indicated. HRP conjugated anti-rat IgG secondary antibody and HRP substrate was used to develop the bands. Signals were detected by enhanced chemiluminescence. No band was detected on the C-terminal deleted mutant of PTMA (lane 3), while bands corresponding to full-length tagged recombinant PTMA were detected with different intensities across the clones