Figure 2

Co-localization of HSP20 and actin but no co-localization of HSP20 and STY013, a nuclear stain, in swine carotid arteries. A (top left). Representative micrograph shows partial co-localization of actin (green) and HSP20 (red). B (top right). An colocalization plot of staining intensities is shown at right and some regions containing HSP20 (horizontal axis) also contained actin (vertical axis). The presence of pixels in the center and upper right comer indicates that some of the HSP20 and actin colocalized. C (bottom left). Representative micrograph shows no co-localization of STY013, a nuclear stain (green) and HSP20 (red). D (bottom right). A colocalization plot of staining intensities is shown at right and demonstrates that regions containing HSP20 (horizontal axis) staining did not stain for the nucleus (vertical axis). E. Reference for colocalization plots; color indicates number of colocalized pixels. Since these studies were performed on intact smooth muscle tissues with high concentrations of HSP20 and actin, imaging would not be expected to detect individual filaments. Each image is 190 microns wide.