Figure 5From: Functional analysis of a frame-shift mutant of the dihydropyridine receptor pore subunit (α1S) expressing two complementary protein fragmentsEC coupling generated by two complementary fragments of α1S. Line scans (horizontal dimension is 2.05 seconds) of fluo-4 fluorescence show Ca2+ transients in response to the indicated 50-ms depolarization from a holding potential of -40 mV. Trace of integrated fluorescence in ΔF/Fo units is shown for each line scan. Each set of depolarizations is from a separate dysgenic myotube expressing the α1S construct(s) indicated at the top of each column. A 16-color calibration bar in ΔF/Fo units is included in Fig. 3 for visual reference.Back to article page