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Figure 1 | BMC Physiology

Figure 1

From: Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells

Figure 1

RT-PCR showing the expression of CFTR in mouse aorta endothelial cells A) cDNA from murine TRP4 +/+ and TRP4 -/- MAEC (lanes 1 and 2), human umbilical vein cells (HUVEC, lane 3) and human nasal epithelium cells (+, for a positive control) were amplified using primers P.CFTR-3249.5 and P.CFTR-3428.3, generating a 180 base pair fragment encoding partial sequences of exons 17a and 17b. Lane 5 is a negative control. B) cDNA from murine TRP4 +/+ and TRP4 -/- MAEC (lanes 1 and 2), from three different preparations of cDNA from HUVEC cells (lanes 3, 5 and 6) and human nasal epithelial cDNA as a positive control (lanes 4 and 7) were amplified using primers PF.CFTR-661.5 and P.CFTR-1360.3, generating a 700 bp fragment harboring exons 5 through 9. Lane 8 is the negative control of amplification.

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