Figure 4

Temporal analysis of [Ca²⁺] _i variations induced by L-glutamic acid in GL15 phenotypes. Of the undifferentiated cells (n = 60), 83% were responsive to addition of 300 μM L-glutamic acid (L-glu) (arrow) with the same kinetics although with different amplitudes (A). In panel B the traces represent the three equally probable [Ca²⁺]_i kinetics (fast increase and fast decay; fast increase and slow decay; Ca²⁺ waves) recorded in 100% (n = 70) of differentiated cells tested. Time (s=seconds) is indicated on the abscissa; the ordinate gives the normalized fluorescence value (f/f₀).