Figure 9
Immunoblot analysis of cx43 protein expression in GL15 cell line 40 μg of total protein extracts from undifferentiated and differentiated GL15 monolayers are electrophoresed and blotted according to Material and Methods. Normal rat heart and IAR 203 rat liver epithelial cells are included as positive controls for cx43 expression. Molecular-mass markers are shown in kDa. All the GL15 cultures express cx43. Note that, in both the rat heart and the IAR 203 samples, all the non-phosphorylated (42 kDa) and the phosphorylated (44 and 47 kDa) isoforms of cx43 are detected by the anti-serum. The protein extracts used for each lane are, (A) rat heart, (B) undifferentiated sub-confluent GL15 cells, (C) undifferentiated confluent GL15 cells, (D) differentiated sub-confluent GL15 cells, (E) differentiated confluent GL15 cells, and (F) IAR 203 cells. Results are representative of three immunoblots.