Nucleus typology | Differentiation days |
|---|
| Â | 0 | 3 | 6 | 9 | 12 |
|---|
normal | 86 ± 10.2 | 72 ± 8.3 | 91 ± 7.5 | 87 ± 9.4 | 94 ± 11.3 |
mitotic | 9 ± 2.3 | 12 ± 2.1 | 2 ± 0.8 | 2 ± 1.5 | 1 ± 0.9 |
apoptotic | 5 ± 1.5 | 16 ± 3.1 | 7 ± 2.6 | 8 ± 2.3 | 5 ± 1.9 |
- Data derived from DAPI-stained nuclei (see Materials and Methods) observed using a fluorescence microscope. Each value represents the mean ± s.d. of the percentage of nuclei with different morphology: regular lobate nuclei (normal), two distinct nuclear lobes (mitotic) and enriched pluri-lobate nuclei (apoptotic). During selected differentiation times (0, 3, 6, 9 and 12 days), the percentages were calculated from values derived from five randomly selected fields each containing about 50-60 cells on slices, in duplicate for each sample.
