Analysis of mitochondria in human pigment retinal epithelial cells exposed to xanthurenic acid. Panels a-c (magnification 200-fold), Cytochrome C release in the presence of 20 μM xanthurenic acid for one week. a, Staining for cytochrome C. b, Hoechst staining. c, Merge of both. Panels d-f (magnification 800-fold), Mitochondria were stained with Mitotracker CMXRos, and cells were stained with antibody against gelsolin (N-half). d, Control cells, e, Cells exposed to 5 μM xanthurenic acid (see Methods), f, Cells exposed to 20 μM xanthurenic acid. Lack of staining with Mitotracker CMXRos, co-staining for cytochrome C (orange), and N-half of gelsolin (green). Panels g-h (magnification 4000-fold), g, Single control mitochondrion stained for cytochrome C. h, Single mitochondrion stained with Mitotracker CMXRos. i, Same as in j, but staining with Mitotracker CMXRos. j, Staining for cytochrome C in cells exposed to 20 μM xanthurenic acid.