Figure 4

Confocal images of the anti-TH IgG treated CNS. TH-IR neurons are indicated with arrowheads. a: the CNS without cerebral ganglia. The giant dopaminergic neuron (LPeD1) is in the left pedal ganglion. TH-IR axons are visible in the ganglia and some nerves. A thick LPeD1 axon is well defined. It passes through the left pleuro-pedal and pleuro-parietal connectives giving off branches and leaving the left parietal ganglion in left parietal nerve (1 pm). b: the CNS with a part of the cerebral ganglia A TH-IR neuron is present in the left parietal ganglion. TH-IR axons are clearly visible in the ganglia and some nerves. c: the CNS without cerebral ganglia. The giant dopaminergic neuron (LPeD1) is in the left pedal ganglion. TH-IR axons are clearly visible in the ganglia and some nerves. A symmetrical group of three TH-IR neurons is clearly visible in the right pedal ganglion. A single TH-IR neuron is present in the visceral ganglion. The structures shown are CG-cerebral ganglia, Pd-pedal ganglia (L-left, R-right), Pl-pleural ganglia (L-left, R-right), Pr-parietal ganglia (L-left, R-right), V-visceral ganglion, cc-cerebral connective, cpc-cerebropedal connective, in-intestinal nerve, pn – pedal nerve. Scale bar = 100 μm.