Figure 4

Transcription of col1a (A-C) and osteocalcin (D-G) in parasagittal sections of phenotypically normal vertebrae from 15 g salmon reared at low intensive (l.i.) and high intensive (h.i.) temperature intensive regime. A. Col1a staining of a large section from l.i. covering 2 vertebrae shows that col1a mRNA (arrows) is highly expressed in osteoblasts lining the growth zone on the endbones of the vertebral bodies and the trabeculae. B. Higher magnification of the endbones; C. The corresponding area in h.i., notice weaker staining. D. Osteocalcin expressed in l.i. at the symmetrical growth zones of 2 adjacent vertebrae; E. The corresponding area in h.i.; F. L.i. Transcription in trabeculae; G. The corresponding area in h.i. Arrows indicate positive staining. Ventral side to the left. Scale bar A:200 μm, B-G:100 μm.