Organization of the midgut junction region. A) The anterior midgut is at the top and the acidic region at the bottom. In green, individual Midgut Junction DH31 expressing cells labelled with ChaGal4 CD8GFP; in red, actin labelled with Phalloidin Alex 546. The Thin arrows denote the insertion of the muscular tethers within the junction region of the anterior midgut. The arrows heads show the constraint of the lumen within the junction region of the midgut outlined with a dotted line. Also note the yeast auto fluorescence in the lumen as well. Size bar is 50 um. B) Labelling with anti-Discs Large (in red) shows a remnant of the tether (arrow) inserting into the musculature of the anterior midgut junction region. In green (UAS CD8GFP/DJ752 Gal40) Midgut Junction DH31 expressing cells are shown. The size bar is 20 um. C) Within the modified longitudinal musculature tether are mono-nucleated (thin arrow) and contain plaques of the Discs Large Protein arranged lengthwise (arrows). The size bar is 20 um.