Figure 2

Schematic representation of the experimental protocol used to induce extracellular dehydration. 4 h (dark gray bar) indicates an acute water and Na⁺ deficit created by two injections of furosemide (0 h, inj. 1 and 2 h, inj. 2, respectively). 24 h (light gray bar) indicates a sustained Na⁺ deficit and rehydration created by ad libitum access to tap water and Na⁺ deficient food. Urine volume and water intake were measured at 4 and 24 h.