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Figure 4 | BMC Physiology

Figure 4

From: Increased expression and local accumulation of the Prion Protein, Alzheimer Aβ peptides, superoxide dismutase 1, and Nitric oxide synthases 1 & 2 in muscle in a rabbit model of diabetes

Figure 4

PrP, Aβ peptides, SOD1, NOS1 and NOS2 protein deposits accumulate in muscle fibers in diabetic rabbits. Above: Immunohistochemical peroxidase detection of A: Aβ peptides (mAb 6E10 Covance), B, C: PrP (rabbit mAb, Epitomics), D: NOS1 (mAb, BD Biosciences), E: NOS2 (mAb, BD Biosciences), F: Superoxide dismutase 1 (Calbiochem), G: No primary antibody, normal control, H: No primary antibody, diabetic muscle. Below: Immunofluorescence detection of proteins in diabetic muscle fibers. A: Aβ (mAb 4G8, Covance), B: PrP (mAb, Epitomics), C: overlay Aβ & PrP staining, D: Brightfield micrograph of degenerating muscle fiber showing vacuoles around the perimeter, E: Fluorescence photograph of anti-SOD1 staining in the same section as D, F: no primary antibody. Muscle fiber cross-sections are ~40-50 μm in diameter. Images are representative of 3 diabetic and 3 control animals.

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