Effects of calorie restriction on body weight and plasma TG level. Body weight is significantly decreased by CR for 2 weeks in both male (A) and female (B) zebrafish. Values are means ± SEM. n = 7 for each group. The plasma TG level is significantly decreased by 2 weeks of CR in male (C) and female (D) zebrafish. Values are means ± SEM. n = 7 for each group. Statistical analyses were performed using Student's t-test to compare the OF8W and the OF8W+CR2W groups. *P < 0.05, **P < 0.01.