Figure 4

Network for lipid metabolic pathways. The network shows direct interactions between the five key regulatory molecules (SREBP1, PPARα/γ, NR3H1 and LEP, shown in yellow) identified by SNEA (Table 3) and their target genes involved in the lipid metabolic pathways (fatty acid metabolism, cholesterol efflux and triglyceride metabolism) identified by GSEA (Table 2). Red and green denote genes with increased and decreased expression, respectively, in obese (OF8W) compared with control (OF1W) zebrafish. Gray denotes genes that were not spotted in the microarray. To reduce the complexity, the rat, mouse and human networks were constructed using the five regulatory factors and their target genes that were spotted in the Agilent Zebrafish Oligoarray. A. zebrafish DIO (GSE18566), B. rat DIO (GSE8700), C. mouse DIO (GSE11790), D. human obesity (GSE15524).