Figure 5

Effects of CR on the coagulation cascade and lipid metabolism in DIO zebrafish. A. Network showing direct interactions between the three key regulatory molecules (IL-6, IL-1β and APOH, shown in yellow) identified by SNEA (Table 3) and their target genes in the coagulation cascade (blood coagulation and platelet activation) identified by GSEA (Table 2). B. Network showing direct interactions between the five key regulatory molecules (SREBP1, PPARα/γ, NR3H1 and LEP, shown in yellow) identified by SNEA (Table 3) and their target genes involved in the lipid metabolic pathways (fatty acid metabolism, cholesterol efflux and triglyceride metabolism) identified by GSEA (Table 2). Red and green denote genes with increased and decreased expression, respectively, at CR2W compared with OF1W. Gray denotes genes that were not spotted in the microarray.