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Figure 2 | BMC Physiology

Figure 2

From: The effects of acylation stimulating protein supplementation VS antibody neutralization on energy expenditure in wildtype mice

Figure 2

Plasma antibody, IL-6, CRP and indirect calorimetry results for mice treated with ASP-neutralizing antibody or control antibody. Fasting plasma samples from NI-IgG treated mice (n = 6, white bars) and Anti-ASP treated mice (n = 7, black bars). (A) Polyclonal rabbit antibody detected in mouse plasma following 9 days and 4 weeks treatment. (B) Plasma IL-6 and (C) CRP levels at end of treatment. Results were analyzed by unpaired two-tailed t-test. (D) Energy expenditure measured over 24 hours for NI-IgG treated mice (n = 6, white squares) and Anti-ASP treated mice (n = 7, black squares). Results were analyzed by two-way ANOVA. Group and time P values are reported in graph, their interaction was not significant. Analysis of light and dark periods separately (2-way ANOVA) indicated a significant difference between Anti-ASP and NI-IgG for both time periods (P < 0.0001) (E) Light and dark phase energy expenditure calculated as the area-under the curve (AUC). (F) respiratory quotient (RQ) measured over 24 hours for NI-IgG treated mice (white squares) and Anti-ASP treated mice (black squares). Results were analyzed by two-way ANOVA. Group and time P values are reported in graph, their interaction was not significant. All values are presented as mean ± SEM, where * P < 0.05.

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