Figure 5

Indirect calorimetry results for rASP treated mice. Energy expenditure (A) and respiratory quotient (C) were monitored continuously over 24 hours for PBS (n = 6, white circles) and rASP (n = 5, black circles) treated mice. Results were analyzed by two-way ANOVA. Group and Time P values are reported in graph, their interactions were not significant. Analysis of energy expenditure in light and dark periods separately (2-way ANOVA) indicated a significant difference between Anti-ASP and NI-IgG for both time periods (both P < 0.0001), and the calculated area-under the curve (AUC) is shown in (B). All values are presented as mean ± SEM, where * P < 0.05, ** P < 0.01.