Figure 3

Diet dependent hypoxic survival. The survival of chronically hypoxic flies fed on different diets was monitored. Flies were fed with liquid diets provided by capillaries under the same conditions as feeding assays. A. Selected survivorship curves. Data were compared using the log rank test. (1) p < 0.0001 as compared to the water only condition, meaning that exogenous sucrose favored hypoxic survival. P < 0.0001 as compared to 10S10Y and 10S-10Y conditions, meaning that exposing flies to yeast decreased hypoxic survival. (2) p < 0.0001 as compared to water only, meaning that these flies did not die of starvation and that a yeast exposure was toxic to the flies. P = 0.0001 as compared to the 10S10Y condition, meaning that ingestion of sucrose limited yeast toxicity. (3) p = 0.0001 as compared to the 10S10Y condition, meaning that self selection conditions (and a reduced yeast intake) improved hypoxic survival. B, C. Survival after 72 hours of chronic hypoxia. The horizontal line and the grey bars indicate the survival of starving flies maintained on a source of water. Means ± sem are indicated. B. (1) p < 0.01 as compared to water only, p < 0.01 as compared to the 5S-5Y and 5S5Y conditions. (2) p < 0.01 as compared to all other conditions. (3) not different from the 5S-5Y condition. C. (1) p < 0.01 as compared to water only. (2) p < 0.01 as compared to all other conditions. (3) p < 0.01 as compared to the 10S10Y condition. Analysis of mortality data after 72 hours of hypoxia was consistent with the analysis of whole survival curves such as those presented in panel A. The number of flies used was 68-138.