Table 1 Details of primer sequences used in qPCR analysis
From: The actions of exogenous leucine on mTOR signalling and amino acid transporters in human myotubes
Gene of interest | Genbank Accession Numbers | Sequences of primers | Amplicon length (bp) |
|---|---|---|---|
Cyclophilin | NM_021130.3 | FP: 5' CATCTGCACTGCCAAGACTGA 3' | 72 |
| Â | Â | RP: 5' TTCATGCCTTCTTTCACTTTGC 3' | Â |
SNAT2 | NM_018976.4 | FP: 5' TGCTGTGCCAATTCTGATCTTT 3' | 185 |
| Â | Â | RP: 5' TGAGACTATGACGCCACCAACT 3' | Â |
LAT1 | NM_003486.5 | FP: 5' AGGAGCCTTCCTTTCTCCTG 3' | 181 |
| Â | Â | RP: 5' CTGCAAACCCTAAGGCAGAG 3' | Â |
LAT4 | NM_152346.1 | FP: 5' TCTCTCCGTGCTCATCTTCA 3' | 165 |
| Â | Â | RP: 5' ATTCCTGGAAAGGTGACTGC 3' | Â |
CD98hc | NM_002394.5 | FP: 5' GCAGATCGACCCCAATTTTG 3' | 79 |