Figure 5From: Receptors and effects of gut hormones in three osteoblastic cell linesP1NP levels after treatments with gut hormones in three osteoblastic cell lines. Cells were serum deprived and treated for 48 or 120 h with the gut peptides at indicated molar concentrations. Straight lines solid square markers designate 48 h treatment; dashed lines emptied square markers indicate 120 h treatment. Significant higher production of P1NP after 120 h exposure to GIP and OB were observed only in Saos-2 (C, O). The least mature cell lines, MG-63 and TE-85 showed decreased P1NP secretion in response to GIP (A), GLP-1 (D, E), GLP-2 (H) and OB (N) stimulation after 120 h exposure. In all the cases the most significant changes were observed with the lowest concentration of peptide. Results are the average of percentage of change in relation to controls; n = 5. ± SEM.Back to article page