Table 1 Details of primer sequences used in qPCR analysis
From: Receptors and effects of gut hormones in three osteoblastic cell lines
Gene of interest | Accesion numbers | Sequence of primers | Product size | Annealing temp (°C) |
|---|---|---|---|---|
COL1A2 | NM_000089 | 5' GGCACTCCAGGTCCTCAG 3' 5' CCACAGCACCAGCAACAC 3' | 100 | 60 |
ALP | NM_000478 | 5' GCTGAACAGGAACAACGTGA3' 5' TCAATTCTGCCTCCTTCCAC 3' | 117 | 60 |
OPG | NM_002546 | 5'GCAGCGGCACATTGGACATG 5'AGGATCTGGTCACTGGGTTTGC3' | 135 | 60 |
β-ACTIN | NM_001101 | 5' GGACCTGACTGACTACCTC 3' 5' GCCATCTCTTGCTCGAAG 3' | 135 | 60 |
GIPr | NM_000164 | 5' GACCAAAGGCTCATCTTGGA 3' 5' ATGTAGCCGCCTGAACAAAC 3' | 114 | 60 |
GLP-1r | NM_002062 | 5' TGGACCAGGAACTCCAACAT 3' 5' TTTGGATACCACGATGCAGA 3' | 114 | 62.5 |
GLP-2r | NM_004246 | 5' TTCCTTTATTGGGCGTTCA 3' 5' CTCTCCATTGGCAAAACCA 3' | 155 | 60 |
GHSr 1a | NM_198407 | 5' GCACTCTTCGTGGTGGGCAT 3' 5' GATGAGCAGATCGGAGAAGG 3' | 123 | 60.5 |
GPR39 | NM_001508 | 5' GCTCATGAAAAGCCAGAAGG 3' 5' CATGATCCTCCGAATCTGGT 3' | 172 | 60 |