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Figure 1 | BMC Physiology

Figure 1

From: Metabolomic phenotyping of a cloned pig model

Figure 1

NMR spectra and assignments. Representative 1H NMR spectra of bile, plasma and urine obtained from a cloned pig. All spectra were acquired on a 600 MHz spectrometer. Less abundant amino acids (Glu, Gln, Lys, and Arg) with complex spectra could not be unambiguously assigned in the spectral region 1.6 - 2.6 ppm in urine. Assignments: 1, bile acids, cholesterol; 2, lipids (-CH2-); 3, bile acids cholesterol, lipids; 4, bisallyllic protons; 5, conjugated taurine; 6, choline or phosphatidylcholine (PC) (-N-(CH3)3); 7, glycine/taurine conjugated cholate (CA), glycine/taurine conjugated chenodeoxy cholate (CDCA); 8, Conjugated deoxycholate (H-3β), conjugated taurine (H-25); 9, PC (CH2-N); 10, Conjugated glycine (H-25); 11, conjugated cholate and deoxycholate, PC-glycerol (3-CH2); 12, PC-glycerol (1-CH); 13, PC (-O-CH2-); 14, CH = CH, cholesterol (6-CH), PC-glycerol (2-CH); 15; Conj bile acids (-NH-); 16, LDL/VLDL; 17, leucine; 18, valine; 19, isoleucine; 20, lactate; 21, alanine; 22, adipate; 23, acetate; 24, N-acetyl glycoproteins; 25, O-acetyl glycoproteins; 26, glutamine/glutamate; 27, pyruvate; 28, glutamate; 29, creatine; 30, choline; 31, trimethylamine-N-oxide (TMAO); 32, glucose; 33, creatinine; 34, tyrosine; 35, phenylalanine; 36, isovaleraldehyde; 37, α-hydroxyisobutyrate; 38, unknown; 39, citrate; 40, dimethylamine; 41, trimethyl amine; 42, taurine; 43, glycine; 44, phenylacetylglycine; 45, hippurate; 46, unknown; 47, alantoin; 48, urea; 49, unknown; 50, guanine. For further information about identified metabolites, please refer to Additional file 1

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