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Table 1 Inward and outward wholecell patch clamp currents, conductances and reversal voltage under established cationic conditions.

From: Sodium selectivity of Reissner's membrane epithelial cells

  

I(-100) [pA]

g(-) [nS]

Vr [mV]

I(+100) [pA]

g(+) [nS]

Series 1

Physiological

[Pipette]/[Bath]

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

 

140KCl/150NaCl

-1305

{277 (7)}

-671 ‡

{146 (7)}

17.2

{2.1 (7)}

10.2 ‡

{1.1 (7)}

9.4

{3.7 (7)}

-2.0 ns

{5.7 (7)}

938

{367 (7)}

708 ns

{257 (7)}

9.9

{3.7 (7)}

7.2 ns

{2.3 (7)}

Series 2

Na or K Bath

150Na-ms/150Na-ms

-2044

{552 (6)}

-255 ‡

{57 (6)}

25.9

{7.0 (6)}

2.6 ‡

{0.5 (6)}

0.5

{0.5 (6)}

-0.9 ns

{1.0 (6)}

1496

{363 (6)}

769 ‡

{177 (6)}

16.5

{3.8 (6)}

13.9 ns

{3.2 (6)}

 

150K-ms/150K-ms

-476‡

{112 (8)}

-429 ns

{130 (8)}

6.1 ‡

{1.3 (8)}

4.6 ns

{1.3 (8)}

-3.8 ‡

{1.2 (8)}

-3.6 ns

{1.2 (8)}

677 ns

{218 (8)}

625 ns

{218 (8)}

8.0 ns

{2.5 (8)}

6.8 ns

{2.4 (8)}

Series 3

Benzamil Na/Li

150Na-ms/150Na-ms

-1922

{96 (5)}

-639 ‡

{151 (5)}

19.8

{1.1 (5)}

5.9 ‡

{2.1 (5)}

0.7

{0.4 (5)}

-0.3 ‡

{0.5 (5)}

2109

{153 (5)}

1413 ‡

{165 (5)}

23.7

{1.6 (5)}

20.3 ‡

{1.7 (5)}

 

150Na-ms/150Li-ms

-1656

{126 (5)}

-682 ‡

{86 (5)}

15.9

{1.3 (5)}

6.5 ‡

{0.7 (5)}

6.3

{1.5 (5)}

3.1 ‡

{1.5 (5)}

1619

{224 (5)}

1203 ‡

{144 (5)}

18.9

{2.6 (5)}

16.8 ns

{1.8 (5)}

  

Benz (-)

Benz (-)

Benz (-)

Benz (-)

Benz (-)

Series 4

Na - Li

150Na-ms/150Na-ms

-1416

{181 (5)}

14.1

{1.6 (5)}

0.1

{0.4 (5)}

1710

{218 (5)}

18.3

{2.1 (5)}

 

150Na-ms/150Li-ms

-1699 ‡

{149 (5)}

16.2 ‡

{1.4 (5)}

7.1 ‡

{1.2 (5)}

1840 ns

{148 (5)}

21.3 ns

{1.5 (5)}

  

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Benz (-)

Benz (+)

Series 5

All cations - benzamil

15Na-ms/150Na-ms

-3434

{363 (5)}

-741 ‡

{65 (5)}

31.2

{3.3 (5)}

6.1 ‡

{0.9 (5)}

29.8

{1.0 (5)}

21.1 ‡

{2.5 (5)}

1666

{243 (5)}

1039 ‡

{162 (5)}

25.7

{3.1 (5)}

21.1 ‡

{2.4 (5)}

  

Benz (-)

Benz (-)

Benz (-)

Benz (-)

Benz (-)

 

15Na-ms/150NMDG-ms

-802 ‡

{118 (5)}

11.3‡

{1.4 (5)}

-27.8 ‡

{3.6 (5)}

1834 ns

{342 (5)}

17.0 ‡

{3.0 (5)}

  1. Table entries are means on the top line and {SEM (number of experiments)} on the second line. Benz, benzamil; Benz(-), without benzamil; Benz(+), with benzamil (1 μM).
  2. ms, Methanesulfonate, The number in front of chemical formula at [Pipette]/[Bath] is concentration (mM).
  3. I(-100) and g(-), whole-cell patch clamp current and slope conductance at -100 mV command voltage; Vr, reversal voltage; I(+100) and g(+), current and conductance at +100 mV command voltage.
  4. Significant differences: ‡, P < 0.05; ns, not significant.
  5. Comparisons:
  6. Series 1, 2, 3 and 5(Na +)--Values in benzamil vs without benzamil (paired); Series 4--Values in Li+ vs Na+; Series 5(NMDG+)-- NMDG+ vs Na+ (both in the absence of benzamil). In series 2, the symbols in the "Benz(+)" column report the significance of changes in values between ±benzamil using the paired t-test and the symbols in the "Benz(-)" column report the significance of changes in values between K+ and Na+ using the unpaired t-test.
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BMC Physiology

ISSN: 1472-6793

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