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Table 1 Inward and outward wholecell patch clamp currents, conductances and reversal voltage under established cationic conditions.

From: Sodium selectivity of Reissner's membrane epithelial cells

   I(-100) [pA] g(-) [nS] Vr [mV] I(+100) [pA] g(+) [nS]
Series 1
Physiological
[Pipette]/[Bath] Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+)
  140KCl/150NaCl -1305
{277 (7)}
-671 ‡
{146 (7)}
17.2
{2.1 (7)}
10.2 ‡
{1.1 (7)}
9.4
{3.7 (7)}
-2.0 ns
{5.7 (7)}
938
{367 (7)}
708 ns
{257 (7)}
9.9
{3.7 (7)}
7.2 ns
{2.3 (7)}
Series 2
Na or K Bath
150Na-ms/150Na-ms -2044
{552 (6)}
-255 ‡
{57 (6)}
25.9
{7.0 (6)}
2.6 ‡
{0.5 (6)}
0.5
{0.5 (6)}
-0.9 ns
{1.0 (6)}
1496
{363 (6)}
769 ‡
{177 (6)}
16.5
{3.8 (6)}
13.9 ns
{3.2 (6)}
  150K-ms/150K-ms -476‡
{112 (8)}
-429 ns
{130 (8)}
6.1 ‡
{1.3 (8)}
4.6 ns
{1.3 (8)}
-3.8 ‡
{1.2 (8)}
-3.6 ns
{1.2 (8)}
677 ns
{218 (8)}
625 ns
{218 (8)}
8.0 ns
{2.5 (8)}
6.8 ns
{2.4 (8)}
Series 3
Benzamil Na/Li
150Na-ms/150Na-ms -1922
{96 (5)}
-639 ‡
{151 (5)}
19.8
{1.1 (5)}
5.9 ‡
{2.1 (5)}
0.7
{0.4 (5)}
-0.3 ‡
{0.5 (5)}
2109
{153 (5)}
1413 ‡
{165 (5)}
23.7
{1.6 (5)}
20.3 ‡
{1.7 (5)}
  150Na-ms/150Li-ms -1656
{126 (5)}
-682 ‡
{86 (5)}
15.9
{1.3 (5)}
6.5 ‡
{0.7 (5)}
6.3
{1.5 (5)}
3.1 ‡
{1.5 (5)}
1619
{224 (5)}
1203 ‡
{144 (5)}
18.9
{2.6 (5)}
16.8 ns
{1.8 (5)}
   Benz (-) Benz (-) Benz (-) Benz (-) Benz (-)
Series 4
Na - Li
150Na-ms/150Na-ms -1416
{181 (5)}
14.1
{1.6 (5)}
0.1
{0.4 (5)}
1710
{218 (5)}
18.3
{2.1 (5)}
  150Na-ms/150Li-ms -1699 ‡
{149 (5)}
16.2 ‡
{1.4 (5)}
7.1 ‡
{1.2 (5)}
1840 ns
{148 (5)}
21.3 ns
{1.5 (5)}
   Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+) Benz (-) Benz (+)
Series 5
All cations - benzamil
15Na-ms/150Na-ms -3434
{363 (5)}
-741 ‡
{65 (5)}
31.2
{3.3 (5)}
6.1 ‡
{0.9 (5)}
29.8
{1.0 (5)}
21.1 ‡
{2.5 (5)}
1666
{243 (5)}
1039 ‡
{162 (5)}
25.7
{3.1 (5)}
21.1 ‡
{2.4 (5)}
   Benz (-) Benz (-) Benz (-) Benz (-) Benz (-)
  15Na-ms/150NMDG-ms -802 ‡
{118 (5)}
11.3‡
{1.4 (5)}
-27.8 ‡
{3.6 (5)}
1834 ns
{342 (5)}
17.0 ‡
{3.0 (5)}
  1. Table entries are means on the top line and {SEM (number of experiments)} on the second line. Benz, benzamil; Benz(-), without benzamil; Benz(+), with benzamil (1 μM).
  2. ms, Methanesulfonate, The number in front of chemical formula at [Pipette]/[Bath] is concentration (mM).
  3. I(-100) and g(-), whole-cell patch clamp current and slope conductance at -100 mV command voltage; Vr, reversal voltage; I(+100) and g(+), current and conductance at +100 mV command voltage.
  4. Significant differences: ‡, P < 0.05; ns, not significant.
  5. Comparisons:
  6. Series 1, 2, 3 and 5(Na +)--Values in benzamil vs without benzamil (paired); Series 4--Values in Li+ vs Na+; Series 5(NMDG+)-- NMDG+ vs Na+ (both in the absence of benzamil). In series 2, the symbols in the "Benz(+)" column report the significance of changes in values between ±benzamil using the paired t-test and the symbols in the "Benz(-)" column report the significance of changes in values between K+ and Na+ using the unpaired t-test.