Localization of HOXA4 protein in cultured human aortic ECs (A) and SMCs (B) using immunofluorescence. The cells were stimulated for 1 or 18 hours using 50 ng/ml IFN-γ. Green, staining with anti-HOXA4 antibody; and Red, F-actin filaments stained with phalloidin. Scale bar = 20 μm. See Additional file 6, Figure S2 for images with DAPI stain.