Table 3 Primers used for RT-PCR and qRT-PCR of ion transporter genes
From: Identification of uterine ion transporters for mineralisation precursors of the avian eggshell
Gene symbol | RefSeq accession | Forward primer | Reverse primer |
|---|---|---|---|
TPRV6 | XM_416530 | AACACCTGTGAAGGAGCTGGTGAG | TCTGCTGCTTGTTTTGTTGCC |
CALB1 | NM_205513 | CAGGGTGTCAAAATGTGTGC | GCCAGTTCTGCTCGGTAAAG |
ATP2A1 | NM_205519 | AAGGGGGGGTCTTTAAGGATGG | CAAACTGCTCCACCACCAACTC |
ATP2A2 | XM_415130 | GCAGCTTGCATATCTTTTGTGCTG | CATTTCTTTCCTGCCACACTCC |
ATP2A3 | NM_204891 | CAACCCCAAGGAGCCTCTTATC | GGTCCCTCAGCGTCATACAAGAAC |
ITPR1 | XM_414438 | AATGGCAAAAGGCGAGGAAAGC | GGAGCAGCAGCAAGCGGG |
ITPR2 | XM_001235612 | TGAGCATTGTGAGTGGCTTC | GTTGACCTGGCTGTCCAAAT |
ITPR3 | XM_418035 | AGTACAACGTGGCCCTCATC | GTCGTGTCTGCTCTCCATGA |
RYR1 | X95266 | GTTCCTCTGCATCATCGGCTAC | AATTGCTGGGGAAGGACTGTG |
ATP2B1 | XM_416133 | CTGCACTGAAGAAAGCAGATGTTG | GCTGTCATATACGTTTCGTCCCC |
ATP2B2 | XM_001231767 | TTACTGTACTTGTGGTTGCTGTCCC | GGTTGTTAGCGTCCCTGTTTTG |
ATP2B4 | XM_418055 | GCTGGTGAAGTTGTCATCCGTC | TGCTCTGAAGAAAGCTGATGTTGG |
SLC8A1 | NM_001079473 | GGATTGTGGAGGTTTGGGAAGG | CTGTTTGCCAGCTCGGTATTTC |
SLC8A3 | XM_001231413 | GGAGAGACCACAACAACAACCATTC | AGCTACGAATCCATGCCCACAC |
SCNN1A | NM_205145 | GCTTGCCAGAAAACAGTCCCTC | AGTCAGACTCATCCAGGTCTTTGG |
SCNN1B | XM_425247 | ATGGAAGTAGACCGCAGT | GTTGTATGGCAGCACAGT |
SCNN1G | XM_414880 | CAAAAGGCACTTCACCCGTTTC | GGACAATGATCTTGGCTCCTGTC |
ATP1A1 | NM_205521 | GCACAAAGAAGAAAAAGGCGAAGG | GGGTGGAGGTGTAAGGGTATTTG |
ATP1B1 | NM_205520 | TCTGGAACTCGGAGAAGAAGGAG | GACGGTGAGCAACATCACTTGG |
SLC4A4 | XM_420603 | GGAAAGCACCATTCTTCGCC | CCTCCAAAAGTGATAGCATTGGTC |
SLC4A5 | XM_423797 | TGAACGTCTCCGCTACATCCTG | ACTTTATCCACCTGGCTGACTCC |
SLC4A7 | XM_418757 | AAATTGCCAAGTTCGTGGTGG | GCGAAGCAAATGAGAAGTTACGG |
SLC4A9 | XM_001232427 | TCCTGACTGGAGTCTCTGTCTTCC | AGGTGATCTGGCTGGTGTTTTG |
SLC4A10 | XR_026836 | CGCTGATGACAGATGAGGTGTTC | GGTGGTTCTATTCGGATTGTTGG |
KCNJ2 | NM_205370 | CCATTGCTGTTTTCATGGTG | TCCTGGACTTGAGGAGCTGT |
KCNJ15 | XM_425554 | TGAGGGAAGGGAGACTCTGA | GCTTCCATCCTCACTGCTTC |
KCNJ16 | XM_425383 | CATTCCTGTTCTCCCTGGAA | CATTTTAGCCAAGGCTGCTC |
KCNMA1 | NM_204224 | GGGATGATGCGATCTGTCTT | GACAAACCCACAAAGGCACT |
CA2 | NM_205317 | ATCGTCAACAACGGGCACTCCTTC | TGCACCAACCTGTAGACTCCATCC |
CA4 | XM_415893 | GCTAACACATTTTTCCCCCTTCC | CTTTATAGCACATCGCATCAGCC |
CA7 | XM_414152 | GCACAAGTCTTATCCCATTGCC | GCCGTTGTTGGAGATGTTGAGAG |
SLC4A8 | XM_001235579 | AGAAGAAGAAGTTGGACGATGCC | GGTCAGTTCTGTCCTTGCTGTTCTG |
SLC26A9 | XM_425821 | GCCTCTTCGATGAGGAGTTTGAG | CTGACCCCACCAAGAACATCAG |
ATP6V1B2 | XM_424534 | ATTCTCTGCTGCTGGTTTGCCC | CATGGACCCATTTTCCTCAAAGTC |
CFTR | NM_001105666 | AAGAGGGCAGGGAAGATCAACGAG | CGGGTTAGCTTCAGTTCAGTTTCAC |
CLCN2 | XM_423073 | CCTGGACACCAATGTGATGCTG | CACGAAGGTCTTCAGGGTGAGATAC |
CLCN5 | XM_420265 | CGATTGGAGGAGTGCTCTTTAGTC | CAAAAGGATTGATGGAACGCAG |