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Table 2 Real time PCR results

From: High molecular mass proteomics analyses of left ventricle from rats subjected to differential swimming training

Spot

Obs. pI

Exp. pI

Obs. M.M.(kDa)

Exp. M.M.(kDa)

Sequ. Match.

Sequ. not match.

Cover.%

e- value

Swissprot code

Protein

Protein funtion

CG

TG1

TG2

TG3

            

Regul. factor

Regul. factor

p-value

Regul. factor

p-value

Regul factor

p-value

1

9.0

9.4

≈ 120

122

25

84

23.3

0.4

Q8K4V5

High-affinity immunoglobulin gamma Fc receptor I.

Phagocytes process

1.00

absent

-

absent

-

1.01

0.05

2

8.1

9.2

248

34.4

70

239

22.7

0.3

Q3KRD5

Mitochondrial import receptor subunit TOM34.

Translocase of outer membrane 34 kDa subunit associated to energy synthesis.

1.00

absent

-

absent

-

1.32

0.05

3

6.9

5.9

175

105

33

55

37.

0.1

Q60I07

MHC class I-like located near the LRC, 1.

Immunological leukocyte response.

1.00

absent

-

absent

-

1.00

0.01

4

4.3

9.7

≈ 250

227

65

135

32.5

1.0

AAH83554

Magnesium transporter MRS2.

Mg2+mitochondria’s transporter

1.00

1.12

0.05

1.27

0.05

1.53

0.01

5

5.9

5.2

≈ 230

364

63

276

18.6

0.1

Q924V0

Cadherin class 1 receptor.

Immune system.

1.00

absent

-

absent

-

absent

-

6

5.2

5.7

≈ 100

314

72

213

25.3

0.6

BAA18993

N-G,N-G-dimethylarginine dimethylaminohydrolase.

NO synthetases inhibitor.

1.00

absent

-

1.00

0.05

1.01

0.05

7

6.4

9.1

120

127

630

477

56.9

0.08

Q63356.1

α-Myosin heavy chain.

Primary motor of muscle contraction on cardiac cells.

1.00

1.04

0.05

0.93

0.01

1.24

0.05

8

5.5

9.2

193.9

34.4

54

185

22.6

0.4

Q3KRD5

Mitochondrial import receptor subunit TOM34.

Translocase of outer membrane 34 kDa subunit associated to energy synthesis.

1.00

absent

-

1.47

0.05

1.36

0.05

9

6.9

5.2

192.6

107

698

277

71.6

0.04

AAF37622

Glutamyl aminopeptidase.

Rennin-angiotensin catabolism pathway.

1.00

1.30

0.05

1.10

0.05

1.32

0.05

10

7.3

9.8

213.0

82

48

270

15.1

0.09

CAA40164

NADH dehydrogenase, mitochondri al subunit 1.

Catalyzes the first dehydrogenase reaction in the TCA cycle

1.00

1.07

0.05

1.18

0.05

1.50

0.05

11

5.8

9.4

223.1

46

52

36

59.1

0.1

Q60I18

Major histocompatibility complex class 1.

Immunological leukocyte response.

1.00

absent

-

absent

-

1.12

0.05

12

8.3

9.6

224.9

215

76

135

36.0

1.0

P23693

Troponin I, cardiac muscle.

Muscular contraction regulator by its relation with calcium molecules.

1.00

1.19

0.01

1.05

0.05

1.26

0.001

13

5.6

9.2

240.0

34.4

58

185

23.9

1.0

Q3KRD5

Mitochondrial import receptor subunit TOM34.

Associated to energy production.

1.00

absent

-

absent

-

1.42

0.08

14

4.8

9.6

232.2

21

44

167

20.9

2.2

P23693

Troponin I, cardiac muscle.

Muscular contraction regulator by its relation with calcium molecules.

1.00

1.00

0.05

1.00

0.05

1.1

0.05

15

5.5

6.1

249.9

42

26

62

29.5

1.0

Q861Q1

Major histocompatibilitycomplex class 1

Involved in the immunological leukocyte response.

1.00

1.00

0.05

1.02

0.05

1.31

0.05

16

8.6

9.2

261

34.4

73

236

23.6

0.8

Q3KRD5

Mitochondrial import receptor subunit TOM34.

Energy synthesis

1.00

absent

-

absent

-

1.0

0.05

17

4.7

5.2

225

107

66

909

6.8

0.04

AAF37622

Glutamyl aminopeptidase.

Rennin-angiotensin catabolism pathway

1.00

absent

-

1.03

0.05

1.13

0.05

  1. Differential proteins identified by MALDI ToF peptide mass fingerprinting of multiple proteins from rat left ventricles. As a standard, false Discovery rate was calculated as lower than 15%.