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Figure 1 | BMC Physiology

Figure 1

From: The ΔF508-CFTR mutation inhibits wild-type CFTR processing and function when co-expressed in human airway epithelia and in mouse nasal mucosa

Figure 1

Co-expression of increasing amounts of ΔF-CFTR alters the processing of WT-CFTR but not ΔTRL-CFTR in IB3-1 CF epithelial cells. ΔF-CFTR does not influence WT-CFTR in heterologous human HEK293T cells. A. IB3-1 CF bronchial epithelial cells transfected transfected with a fixed amount of WT-CFTR and increasing amounts of ΔF-CFTR. Two different examples are shown, a full titration and a partial experiment imaged by different methods. B. HEK293T cells were transfected with the same mixtures and CFTR detected biochemically. C. The relative effect of ΔF-CFTR on the processing of a fixed amount of ΔTRL-CFTR in the presence of increasing amounts of ΔF-CFTR provided in a typical blot and effect of ΔF-CFTR on both fully processed CFTR constructs (WT and ΔTRL) was quantified and graphed.

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BMC Physiology

ISSN: 1472-6793

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