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Figure 3 | BMC Physiology

Figure 3

From: The ΔF508-CFTR mutation inhibits wild-type CFTR processing and function when co-expressed in human airway epithelia and in mouse nasal mucosa

Figure 3

Co-expression of increasing amounts of ΔF-CFTR alters the function of WT-CFTR in IB3-1 CF epithelial cells but not in HEK293T cells. A. IB3-1 cells are seeded on Vitrogen coated coverslips and transiently transfect in the manner previously described in Figure1. Twenty-four hours after transfection the cells are loaded with the halide sensitive dye, SPQ, overnight. The cells relative fluorescence is then measured while incubated in three different buffers: NaI, NaNO3, and NaNO3 with cAMP agonists, and then back into NaI. B. HEK293T cells were seeded on Vitrogen free coverslips and transfected, loaded, and measured as previously described in A.

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BMC Physiology

ISSN: 1472-6793

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