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Figure 6 | BMC Physiology

Figure 6

From: The ΔF508-CFTR mutation inhibits wild-type CFTR processing and function when co-expressed in human airway epithelia and in mouse nasal mucosa

Figure 6

WT-CFTR function is altered by ΔF508-CFTR in WT-CFTR/ΔF508-CFTR heterozygous MTE A. Mouse tracheal epithelial cells (MTE) were seeded on Vitrogen coated filter inserts as previously described. On day three, the cells are grown in an air/liquid interface: media on the basolateral side but no media on the apical side. The RTE of the MTE monolayers are allowed to reach 1000 Ω·cm2 or above prior to experimentation The MTE monolayer ISC current response to amiloride, forskolin, and genistein are then measured and record ed. B. Summary of the ISC data of the three genotypes of the ΔF508 MTE monolayers. Homozygous cell monolayers failed to respond altogether and the summary ISC response was equal to zero.

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BMC Physiology

ISSN: 1472-6793

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