Skip to main content
Figure 6 | BMC Physiology

Figure 6

From: Localization of lipoprotein lipase and GPIHBP1 in mouse pancreas: effects of diet and leptin deficiency

Figure 6

Ob/ob mice islets lack LPL in beta cells, but the expression was restored after leptin-treatment. (A) Islet from an ob/ob mouse stained with anti-LPL (red), (B) with anti-insulin (green), and (C) merge of A and B. (D) Islet from a leptin-treated ob/ob mouse stained with anti-LPL, (E) with anti-insulin and (F) merge between D and E. (G) Another islet from an ob/ob mouse stained with anti-LPL, (H) with anti-glucagon (green) and (I) merge of G and H demonstrating co-localization between LPL and alpha cells. (J) Islet from a leptin-treated mouse stained with anti-LPL, (K) with anti-glucagon and (L) merge between J and K demonstrating LPL reaction in some areas in addition to those containing alpha cells. (M) LPL mRNA levels in islets from WT (n=10), saline-treated (n=6) and leptin-treated (n=4) ob/ob mice (six months old). Data are presented as mean ± SEM (Kruskal-Wallis p=0.044) and normalized to the results for WT (100%). (N) LPL mRNA expression in ob/ob mice from one to eleven months of age (n=4 analyzed in each age group except at three months, n=3) calculated as relative expression towards beta actin. Values were normalized to one month old ob/ob (100%) and are presented as mean ± SEM (Kruskal-Wallis where the lowest p-value was calculated to 0.057 between three and eleven months).

Back to article page