Figure 3

ChAT–positive fibers are nerve elements. Representative photomicrographs of 12 μm thick cross-sections of the urinary bladder isolated from ChAT mice. Sections were stained with DAPI (4',6-diamidino-2-phenylindole) to highlight the cell nucleus and with class III β-tubulin antibody. A-D illustrates that both β-III tubulin- and ChAT-positive fibers are distributed within the urothelium and detrusor smooth muscle. Areas highlighted by dotted squares in D were photographed at higher magnification to show the overlap of beta-III tubulin(green)- and ChAT(red)- positive fibers fluorescence in the urothelium (E-H) and detrusor smooth muscle (I-L).