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Figure 4 | BMC Physiology

Figure 4

From: VEGF induces sensory and motor peripheral plasticity, alters bladder function, and promotes visceral sensitivity

Figure 4

Relative density of ChAT-positive nerve fibers in comparison with PGP9.5, Substance P (SP), and TRPV1. Mouse urinary bladders were isolated from control ChAT mice and stained for the pan neuronal marker PGP9.5, TRPV1, and SP. The density of immunoreactive fibers was counted in 6-10 non-overlapping cross-sectional images of the urinary bladder (400X magnification). The number of fibers (length between 0.19-500 μm and width 0.19-2.5 μm) stained positively by a particular antibody per cross-sectional area was calculated and the results of 6-10 fields were averaged. This procedure was repeated for 5 bladders and the results are expressed as mean ± SEM.

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BMC Physiology

ISSN: 1472-6793

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