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Figure 8 | BMC Physiology

Figure 8

From: VEGF induces sensory and motor peripheral plasticity, alters bladder function, and promotes visceral sensitivity

Figure 8

VEGF increases the density of cholinergic nerves. ChAT-positive fiber density in the urinary bladder isolated from ChAT mice 1 week after treatment with PBS, or 1 and 2 weeks of after VEGF instillations. The results indicate a significant increase in ChAT density in the urothelium (A), lamina propria (B), and detrusor smooth muscle (C). The density of immunoreactive fibers was counted in 6-10 non-overlapping cross-sectional images of the urinary bladder (400X magnification). The number of fibers (length between 0.19-500 μm and width 0.19-2.5 μm) stained positively by a particular antibody per cross-sectional area was calculated and the results of 6-10 fields were averaged. This procedure was repeated for all bladders and the results are expressed as mean ± SEM. (N=5 per group).

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