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Figure 1 | BMC Physiology

Figure 1

From: Small molecule screening platform for assessment of cardiovascular toxicity on adult zebrafish heart

Figure 1

A) Whole heart, isolated from wild type adult zebrafish showing Atrium (abbreviated: at) and Ventricle (abbreviated: v). B) Petri dish with embedded hearts used for screening. C) Diagrammatical representation of the screening setup. Petri dish with embedded hearts is placed on an inverted microscope having an in- and out-let to regulate the in- and out-flow of fresh Krebs buffer. D) Analyses of heartbeating using MATLAB. E) Three hearts mounted on a Petri dish to measure Ca2+ responses. The regions of interest (ROI1 and ROI2) that were used to analyze Ca2+ responses are shown with blue and red circles. F) Graph showing spontaneous Ca2+ activity and compound-induced Ca2+ response in ROI1 (in blue) and ROI2 (in red). Analyses of heartbeats at 28°C G) and 37°C H). Analyses of heartbeats obtained from screen of compounds as indicated carried out at 28°C I) and 37°C J). Compound concentrations of 10 μM were used in the screen. Scale bar: 200 μm in A.

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