Figure 1

VEGF isoforms, MMP9 , MMP15 mRNA and protein expression in omentum and subcutaneous AT from subjects. (A) VEGF isoforms [VEGF-A, VEGF-B, VEGF-C and VEGF-D] mRNA expression analysis was performed on human omentum (OM) and subcutaneous (SC) adipose tissues from three subject groups (lean, OB/H-IR and OB/L-IR subjects). mRNAs were normalized to cyclophilin levels. Results were obtained from triplicate and expressed as the mean ± SEM (n = 17 for controls, n = 12 for OB/L-IR and n = 14 for OB/H-IR). Bars with different letters have a significant difference, p < 0.05. (B) Immunoblotting analysis of VEGF isoforms. The resulted blot of each isoform was compared to Actin-β constitutive protein. Density analyze was done normalizing the samples to β-actin using NIH's Image-J software program. The blot is representative of three independent experiments with different samples, * p < 0.05. (C) Metalloproteinase (MMP9 and MMP15) mRNA expression analysis was performed on human omentum (OM) and subcutaneous (SC) adipose tissues from three groups of subjects (leans, OB/H-IR and OB/L-IR). mRNAs were normalized to cyclophilin levels. Bars with different letters have a significant difference (p < 0.05); differences are compared to the control and between the two groups of obese.